Establishment and application of multiplex PCR method for detection of Babesia ovata and Theileria sinensis
In order to establish a fast duplex PCR method to simultaneously detect Babesia ovata and Theileria sinensis.The primers which could specificall amplify the sequences of the AMA1of Babesia ovata,and the 16S rRNA of Theileria sinensis.A multiplex PCR was developed to amplify specific DNA fragment of Babesia ovata and Theileria sinensis with the target Sequence of 986 bp and 500 bp.This method was specific in detecting Babesia ovata and Theileria sinensis.The detection limit was 16 fg/μL for Babesia ovata and Theileria sinensis,respectively.Clinical samples from Shulan in jilin province were detected by the multiplex PCR and the detection rate was 30％for Babesia ovata;16.67％for Theileria sinensis and 10％for mixed infection.It showed that the established multiplex PCR is suitable for quickly clinical detection and molecular epidemiological investigation.
国家科技期刊平台
NSTL
万方数据
维普
