Prokaryotic Expression of VP1 Protein of Senecavirus A and Preparation of Its Polyclonal Antibodies
Senecavirus A(SVA)is an emerging swine pathogen that can cause vesicular lesions in sows and acute death of piglets.In this study,the VP1 gene of SVA was cloned into the prokaryotic expression vectors pCold-Ⅰ and pCold-TF and then the recombinant plasmids VP1-pCold-Ⅰ and VP1-pCold-TF were verified and transformed into E.coli BL21(DE3)to induce the expression of recombinant protein.The results showed that VP1-pCold-Ⅰ existed in the precipitates(inclusion bodies)and VP1-pCold-TF was a soluble protein.The purified proteins were used to immunize BALB/c mice.The polyclonal antibodies were obtained after immunization four times and detected in Western blotting and indirect immunofluorescence assay(IFA).The prepared polyclonal antibodies had good tiers in Western blot and IFA,which provided a tool for relevant basic and applied research.
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维普
