Genetic Variation of E0 Gene of Classical Swine Fever Virus from 2018 to 2020 in Fujian Province
In order to investigate the prevalence and genetic variation of classical swine fever virus(CSFV)from 2018 to 2020 in Fujian province,1464 samples of whole blood,lungs,spleens and lymph nodes were collected from clinical sick pigs and detected by RT-nPCR.Then the E0 gene were cloned from the CSFV-positive samples and sequenced for genetic analysis.The results showed that 1.9％samples(19/1464)were CSFV-positive and E0 gene was amplified and sequenced from 13 samples.The homology of E0 gene sequences of these 13 strains ranged from 82.2％to 99.7％,among which 11 strains shared 99.0％to 99.9％homology with the representative strain HCLV of subtype 1.1 and 94.1％to 95.0％with the Shimen strain.Additionally,one strain had 98.2％nucleotide homology with the 2.1c reference strain HNSD-20121and another strain had 99.6％nucleotide homology with CSFV/JPN/2018 subtype 2.1d strain.Genetic evolution analysis showed that 10 strains were in the same branch with HCLV,C-ZJ-2008,Shimen and Brescia strains,belonging to subtype 1.1,one strain that belonged to 2.1c subtype was in the same branch with HNSD-2012and another strain was located in the same branch with CSFV/JPN/2018of2.1d subtype.Amino acid sequence analysis showed that 13 strains were highly conserved at RNase active sites as compared with the representative strains such as HCLV.However,2 strains that belonged to subtype 2.1,had a G→E mutation at the 35th non-critical amino acid residue.These results showed that the CSFV in Fujian province existed in a low prevalence but highly genetic diversity,suggesting a necessity to continuously monitor CSFV genetic variation.
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