Immunological Protection of MIC1-MIC6-Rop18 Recombinant Vaccine Against Toxoplasma gondii in Mice
To evaluate the immune protection of recombinant protein vaccine composed of Toxoplasma gondii microneme proteins 1,6(MIC1,MIC6)and rhoptry protein 18(ROP18)in BALB/c mice,different combinations of those proteins were emulsified with Freund's adjuvant and used to subcutaneously immunize mice.The specific antibodies in mouse serum samples and cytokines levels in the cultural supernatant of splenocytes were tested using ELISA method.After the final immunization,mice were challenged with virulent T.gondii WH3 strain and the survival time was recorded.The results showed that the mice immunized with the recombinant proteins produced higher titers of IgG,IgGl and IgG2a than those in PBS control group and the titer reached above 1:128 000.The levels of IL-4,IL-10 and IFN-y in the immune groups increased to varying degrees as compared with PBS control group.Meanwhile,these recombinant proteins induced specific lymphocyte proliferation.Moreover,the survival time of immunized mice was prolonged than control group.Taken together,the results in the present study indicated that vaccination with the recombinant MIC1,MIC6 and ROP18 proteins elicited strong humoral and cellular immune responses in mice,in which MIC6-ROP18 protein combination had a more obvious immune protection effect but needed further investigation.Our study provided a reference for the development of T.gondii vaccine.
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