Application of High Resolution Melting Curve for Detection of Ofloxacin Resistance of Mycobacterium tuberculosis
To evaluate the effectiveness of high-resolution melting curve(HRM)technology in the detection of ofloxacin(OFLX)resistance,84 Mycobacterium tuberculosis isolates were tested by the drug sensitivity test(DST),HRM and DNA sequencing.The chi-square test and Kappa analysis were used to compare the difference and the consistency of these three methods.The results of DST showed that 12 strains were resistant and 72 strains were sensitive to OFLX.The results of DNA sequencing showed that 10 strains had mutations and 74 strains had no mutations to gyrA gene.The results of HRM showed that 14 strains were resistant and 74 strains were sensitive to OFLX.With the DST results by the proportion method as controls,the sensitivity,specificity and diagnostic consistency rate of DNA sequencing for detection of drug resistance to OFLX was 83.3％,100％and 97.6％,which were highly consistent.The sensitivity,specificity and diagnostic consistency rate of HRM for detection of drug resistance to OFLX was 75％,93.1％and 90.5％,which were moderate consistent.According to the results of DNA sequencing,the sensitivity,specificity and diagnostic consistency rate of HRM for detection of drug resistance to OFLX was 80％,91.9％and 90.4％,which were moderate consistent.Take together,HRM had good sensitivity and specificity for the detection of OFLX resistance in M.tuberculosis,which might be used to assist rapid diagnosis,screening and early treatment.
国家科技期刊平台
NSTL
万方数据
