Drug Resistance and PFGE Analysis of Pasteurella multocida Isolates of Pig Origin in East China from 2017 to 2020
In order to investigate the drug resistance and epidemic characteristics of 31 Pasteurella multocida strains isolated from pigs in East China,minimum inhibitory concentrations(MICs)against 12 commonly used antibiotics were determined by agar dilution method and molecular typing was performed by pulsed field gel electrophoresis(PFGE).Highest resistance rate(61.29％)was observed for sulfamethoxazole,followed by tiamulin(32.26％),florfenicol(25.81％),doxycycline(12.90％)and spectinomycin(3.23％).All of these 31 strains were susceptible to ceftiofur,gentamicin,amoxicillin-clavulanic acid and enrofloxacin.These 31 P.multocida strains showed 8 drug resistance patterns,among which 2 strains exhibited resistance to 3 antibiotics(DOX+TIA+SPT and FFC+TIA+SXT).According to 85％similarity criteria,these 31 P.multocida strains were divided into 14 PFGE types,of which 19 strains(61.29％)clustered in four types,namely A,G,H and J.The antimicrobial resistance profiles of those strains with the same PFGE types were also highly similar.Our results demonstrated that P.multocida strains from pigs in East China showed diverse AMR patterns and PFGE types.There was a certain correlation between PFGE typing and antimicrobial resistance profiles.Monitoring of drug resistance and epidemic prevention and control of P.multocida from pigs should be strengthened in the future work.
国家科技期刊平台
NSTL
万方数据
