摘要
本研究利用PCR从有腹泻症状的鸽中检测到圆环病毒,进一步进行了全基因组扩增.基因组序列分析表明该基因组长度为2032 bp,编码5个开放阅读框(ORFs),5'端和3'端各存在一个基因间隔区.该基因组序列与已登录的中国、澳大利亚、日本、波兰、比利时和巴西的PiCV基因序列同源性为81.5％～97.7％.全基因组系统发育分析显示,54个序列(53个参考PiCV基因组序列和1个本研究PiCV基因组序列)可分为6个分支(A、B、C、D、E和F),本研究分离毒株位于B支,与来自比利时(DQ915957和DQ915958)、中国(KX108826、KX108805、KX108825、KJ704802 和 KJ704806)的分离株亲缘关系密切.Simplot 重组分析结果显示Rep ORFs包含多个重组热点,表明我国鸽圆环病毒存在遗传多样性.
Abstract
In this study,a Pigeon circovirus(PiCV)was detected from a diarrheic racing pigeon in Tianjin,by using PCR method.Sequence analysis of this PiCV strain revealed a genome size of 2032 bp,consisting of 5 open reading frames(ORFs)and 2 intergenic regions located at the 5'and 3'ends.Analysis of the pairwise sequences showed that this Tianjin PiCV strain shared 81.5％-97.7％genomic identity with other PiCVs from China,Australia,Japan,Poland,Belgium and Brazil.Phylogenetic analysis was performed for the full genomes of 53 references and this Tianjin strain by neighbor-joining method.As a result,these 54 PiCV strains were divided into 6 clades(A,B,C,D,E and F).The Tianjin strain was located in the clade B and was closely related to the strains from Belgium(DQ915957 and DQ915958)and China(KX108826,KX108805,KX108825,KJ704802 and KJ704806).Recombination analyses were carried out with Simplot and found that Rep ORFs contained several recombination hotspots,suggesting genetic diversity of PiCVs in China.
基金项目
河北省科技厅重点研发计划(20326624D)
河北省高等学校科学技术研究项目(ZD2020314)
河北省高层次人才资助项目(A202003006)
河北省第二期现代农业产业技术体系蛋肉鸡创新团队建设专项经费(HBCT2018150207)
河北北方学院校级科研项目(YB2020006)
河北北方学院校级科研项目(XT2021008)
国家科技期刊平台
NSTL
万方数据