猪肠病毒G型部分VP1蛋白的原核表达及多克隆抗体制备
Prokaryotic Expression and Polyclonal Antibody Preparation of Partial VP1 Protein of Porcine Enterovirus G
边金妮 1黄诗婷 1许佳乐 1米雪 1王奕斐 1杜琛 1陈樱 1韦祖樟 1黄伟坚 1欧阳康1
作者信息
- 1. 广西大学动物科学技术学院动物传染病与分子免疫学实验室,南宁 530005
- 折叠
摘要
本研究扩增猪肠病毒G型(EV-G)的部分VP1基因,将其克隆至原核表达载体,并对其进行诱导表达,获得重组蛋白并制备多克隆抗体,通过酶联免疫吸附试验,免疫印迹试验和间接免疫荧光试验对其进行验证.结果表明,本研究制备的兔抗EV-G-VP1多克隆抗体具有良好的免疫原性和反应性,可以特异性识别EV-G感染Marc-145细胞表达的VP1蛋白,为猪肠病毒G型免疫学检测方法的建立提供良好的生物学材料.
Abstract
In this study,part of VP1 gene of enterovirus type G(EV-G)was amplified,cloned into prokaryotic expression vector and induced for protein expression.The recombinant protein was obtained preparation of polyclonal antibodies.The reactivity of rabbit anti-EVG-VP1-1 was verified by enzyme-linked immunosorbent assay(ELISA),Western blot and indirect immunofluorescence assay(IFA).The results demonstrated that the polyclonal antibodies not only had effective immunogenicity but also had specific reactivity as they specifically reacted with VP1 protein of EV-G infected Marc-145 cells.The availability of polyclonal antibodies provided valuable biomaterials for development of immunological detection method for EV-G.
关键词
猪肠病毒G型/部分VP1蛋白/原核表达/多克隆抗体Key words
Enterovirus G(EV-G)/partial VP1 protein/prokaryotic expression/polyclonal antibody引用本文复制引用
基金项目
广西自然科学基金资助项目(2021GXNSFAA196067)
出版年
2024
国家科技期刊平台
NSTL
万方数据