Prokaryotic Expression and Polyclonal Antibody Preparation of Partial VP1 Protein of Porcine Enterovirus G
In this study,part of VP1 gene of enterovirus type G(EV-G)was amplified,cloned into prokaryotic expression vector and induced for protein expression.The recombinant protein was obtained preparation of polyclonal antibodies.The reactivity of rabbit anti-EVG-VP1-1 was verified by enzyme-linked immunosorbent assay(ELISA),Western blot and indirect immunofluorescence assay(IFA).The results demonstrated that the polyclonal antibodies not only had effective immunogenicity but also had specific reactivity as they specifically reacted with VP1 protein of EV-G infected Marc-145 cells.The availability of polyclonal antibodies provided valuable biomaterials for development of immunological detection method for EV-G.
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