一株H9N2亚型禽流感病毒抗原变异株产生原因的分析

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国家科技期刊平台
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中文摘要：目前，H9N2亚型禽流感病毒是我国最主要的低致病性禽流感病毒，造成禽养殖业的严重经济损失。H9N2禽流感病毒灭活疫苗是防控该疫病的最有效方式，然而，H9N2病毒在免疫压力下容易产生抗原变异，导致疫苗免疫失败，其机制尚不清楚。在前期研究中，我们通过模拟H9N2禽流感免疫压力筛选出携带HAD201N单点突变的逃逸株，但HAD201N如何导致H9N2病毒逃逸株出现的原因尚不明确。为此，本研究利用8质粒反向遗传技术方法拯救出1株A/Chicken/Shanghai/1/2006(H9N2)(简称SH1)及其免疫逃逸株rSH1-HAD201N。为了鉴定HAD201N是否通过影响H9N2禽流感病毒复制能力而导致病毒发生免疫逃逸，用0。001 MOI的病毒接种MDCK并测定其生长曲线，结果表明HAD201N点突变可显著降低rSH1病毒的复制能力，且病毒复制能力的变化不是rSH1病毒发生逃逸的主要原因。为了研究HAD201N是否通过影响H9N2病毒抗原变异而产生病毒逃逸株，将原毒株与突变毒株加入佐剂后免疫鸡以制备多克隆抗体血清，利用交叉HI试验，计算R值并分析抗原变异，结果表明rSH1与rSH1-HAD201N的R值为2，说明HAD201N会导致rSH1发生显著的抗原变异，从而使病毒在免疫压力下逃逸。本研究为探索H9N2禽流感病毒抗原变异规律及其疫苗研发奠定了基础。

外文标题：Analysis of the Antigen Variation of A H9N2 Avian Influenza Virus Strain

外文摘要：At present,the H9N2 subtype avian influenza virus is the most important low pathogenic variant in China,causing serious economic losses to the poultry industry.The inactivated H9N2 avian influenza virus vaccine is the most effective way to prevent and control the disease.However,the H9N2 virus is prone to antigenic variation under immune pressure,resulting in the failure of vaccine immunity.The mechanism is still unclear.In the previous study,we screened escape strains carrying a single point mutation of HAD201N by simulating the immune stress of H9N2 avian influenza but the reason why HAD201N led to the emergence of H9N2 virus escape strains was still unclear.In the present study,we rescued a strain of A/Chicken/Shanghai/1/2006(H9N2)(rSH1)and its immune escape strain rSH1-HAD201N by using the 8-plasmid reverse genetic technology.In order to identify whether HAD201N caused immune escape of the virus by affecting the replication ability of H9N2 avian influenza virus,MDCK cells were inoculated with the rSH1 virus at 0.001 MOI and its growth curve was measured.The results showed that the point mutation of HAD201N significantly reduced the replication ability of rSH1 virus but the change of virus replication ability was not the main reason for the escape of rSH1 virus.In order to investigate whether HAD201N produced virus escape strains by affecting the antigenic variation of H9N2 virus,the original virus and mutant virus were emulsified with adjuvant to immunize chickens for preparation of antiserum.Cross-HI test was used to calculate the R value and analyze the antigenic variation.The R value of rSH1-HAD201N was 2,indicating that HAD201N caused significant antigenic variation in rSH1,allowing the virus to escape immune stress.This study laid a foundation for further exploring the antigenic variation of H9N2 avian influenza virus and vaccine development.

外文关键词：

H9N2 subtype avian influenza virusreverse genetics technologypoint mutationimmune escape

作者：

乔淑媛、滕巧泱、李雪松、刘芹防、张志飞、王思琪、李泽君、杨健美、王彩云

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作者单位：

内蒙古农业大学兽医学院,呼和浩特 010018

中国农业科学院上海兽医研究所,上海 200241

关键词：

H9N2亚型禽流感病毒反向遗传技术点突变免疫逃逸

基金：

中国农业科学院创新工程基金国家青年科学基金项目上海市科技兴农项目中央级公益性科研院所基本科研业务费专项

项目编号：

CAAS-ASTIP-SHVRI-2014-4317001362021-02-08-00-12-F00746JB03

出版年：

2024

中国动物传染病学报

中国农业科学院上海兽医研究所

中国动物传染病学报

CSTPCD北大核心

影响因子：0.651

ISSN：1674-6422

年,卷(期)：2024.32(4)