Prokaryotic Expression and Membrane Localization of Mycoplasma gallisepticum P31 Protein
In order to investigate the function of Mycoplasma gallisepticum(MG)membrane protein,the transmembrane area 714 bp was analyzed using bioinformatics software and for prokaryotic expression of a target protein with molecular weight about 31 kDa.The expressed recombinant P31 protein was purified and used for preparation of rabbit polyclonal antibodies.The rabbit polyclonal antibodies were titrated to be 1:25 600 by ELISA.The P31 protein was distributed on the surface of MG membrane as visualized in Western blot.In this study,P31 protein was confirmed to be a MG membrane protein with good antigenicity,which could be used as a candidate protein for developing novel MG diagnostic methods,genetic engineering vaccines and therapeutic drugs.
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