Prokaryotic Expression and Antiviral Activity of Canine Interferon Fused with Fe Protein
In this study,ferritin was fused with canine interferon α to obtain specific drugs for canine viral infectious diseases.Firstly,according to the synthesis template of canine interferon α sequence(IFN2a)published in GenBank,the ferritin encoding gene was linked to the upstream by fusion PCR and the recombinant gene was cloned into the prokaryotic expression vector pCold-TF to obtain the recombinant plasmid pCold-TF-CaIFN2a-Fe.After transformation into BL21,the recombinant protein was induced by IPTG and identified by SDS-PAGE and Western blot.The soluble recombinant protein of 89 kDa was obtained and purified with nickel column.Its antiviral activity was detected using MDCK/VSV microcytopathy inhibition assay.The antiviral titer was 8×104 IU/mg,showing a good application prospect.
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