戊型肝炎病毒感染状态下兔粪便菌群的变化探究
Analysis of Changes in Fecal Microbiota in Rabbits with Hepatitis E Virus Infection
李曼郁 1蓝海云 1李克坚 1周诚1
作者信息
- 1. 中国食品药品检定研究院体外诊断试剂检定所传染病诊断试剂一室,北京 100050
- 折叠
摘要
本研究旨在探究HEV感染状态下兔粪便菌群的变化差异.本研究收集粪便HEV RNA阳性与阴性兔粪便各4份,分为P组(n=4,P1～P4)和N组(n=4,N1～N4),对粪便16S rDNA基因序列中的V3V4区域进行高通量测序,并对所得数据进行了生物信息学分析.α多样性及PCoA分析结果显示2组间的粪便菌群组成存在明显差异;与阴性对照组相比,HEV感染组的拟杆菌门、酸杆菌门、绿弯菌门等显著增多(P<0.05),瘤胃球菌属、拟杆菌属等丰度显著减少(P<0.05).HEV感染后兔粪便菌群的构成、丰度等均存在一定差异,粪便菌群有可能成为HEV感染的潜在生物标志物.
Abstract
This study was designed to investigate the changes in fecal microbiota in rabbits with Hepatitis E virus(HEV)infection.A total of 4 fecal samples of HEV RNA positive rabbits and 4 fecal samples of HEV RNA negative rabbits were collected and divided into P and N groups.The next-generation 16S rDNA sequencing was performed and the data was analyzed for these changes.The results of alpha diversity and PCoA analysis showed a significant difference in the compositions of fecal microbiota between the two groups.Compared with the negative control group,Bacteroidota,Acidobacteriota,Chloroflexi and Muribaculaceae in the HEV infection group significantly increased(P<0.05)while Ruminococcus and Bacteroides significantly decreased(P<0.05).These changes in the composition and abundance of fecal microbiota in rabbits with HEV infection might have the potential significance to become a biomarker of HEV infection.
关键词
戊型肝炎病毒/粪便菌群/高通量测序Key words
Hepatitis E virus/fecal microbiota/high-throughput sequencing引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据