Prokaryotic Expression of Truncated BVDV NS5B Protein and Preparation of Polyclonal Antibodies
The purpose of this study was to clone the gene fragment of the non-structural protein NS5B of Bovine viral diarrhea virus(BVDV)for prokaryotic expression of the truncated protein and prepare its polyclonal antibodies.The constructed plasmid pET-28a-NS5B was transformed into BL21 competent cells.The truncated NS5B protein was expressed with induction of IPTG and mainly released as inclusion bodies.The polyclonal antibodies prepared by immunizing mice had good reactivity and specifically recognized the eukaryotic expressed NS5B protein.The expression of truncated NS5B protein and preparation of polyclonal antibodies in this study laid the foundation for further studying the biological function of NS5B protein and developing a serological method for differential diagnosis of BVDV.
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