Cloning and prokaryotic expression of Gal/GalNAc lectin hgl LC3 gene of Entamoeba histolytica isolated from Macaca mulatta
To develop genetic engineering vaccine against Entamoeba histolytica infection in animals,a LC3 segment of hgl gene of E.histolytica was amplified from total genomic DNA of E.histolytica from Macaca mulatta by RT-PCR,and then subcloned into the pET-32b(+)vector.The recombinant vector pET-32b(+)-hglLC3 was transformed into Escherichia coli BL21 for expression with IPTG induction.The LC3 segment of hgl gene of E.histolytica was 1 200 bp.SDS-PAGE analysis indicated that the expressed fusion protein in recombinant E.coli was approximate 66 ku in molecular weight.Western-blot analysis showed that the fusion protein could be recognized by the hyperimmune sera from the rabbits inoculated with E.histolytica proteins,indicating that the recombinant protein expressed from the LC3 segment of hgl gene could be used as a candidate antigen for development of effective vaccines against E.histolytica infection in animals.
国家科技期刊平台
NSTL
万方数据
维普
