Study on the culture and preservation of Caenorhabditis elegans
Growth effect of Caenorhabditis elegans on four different medium including OP50-NGM,JM109-NGM,OP50-LB and JM109-LB were evaluated and compared with each other.The preserving effects of C.elegans in three preserving solutions,with different proportional glycerol were evaluated under the condition of liquid nitrogen-80℃ and-20℃,respectively.Results showed that C.elegans grew better on the NGM medium covered with E.coli OP50,than other three mediums.For preserving solution,C.elegans had a survival rate of 78% in LB medium containing 50mL/L glycerol at-80℃ and it had a survival rate of 52% in PBS at the same condition as LB medium,while in water with 100mL/L glycerol,the survival rate was only 8%.Both adults and larvae can survive in liquid nitrogen,but only the larvae can survive at-80℃and-20℃.In conclusion,C.elegans grew well on NGM medium covered with E.coli OP50,and the optimum preserving condition was LB medium with 50mL/L glycerol at-80℃.
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