Through studying the influence of frankincense extract on repairing peripheral nerve injury,thus offering the theoretical foundation for wound healing of herbs,rat Schwann cells(SCs) were subcultured as a experimental model,and it was cultured with different concentrations of the extract.In the range of safe concentration,SCs were randomly divided into control group and drug group,and different concentration of the extract was added to intervene cell growth.The cell proliferation was observed at hour 12,24,48,72 and 96 post-cultivation.Flow cytometry method was applied to test cell cycle and the percent content of DNA.ELISA kit was used to detect the content of brain derived neurotrophic factor(BDNF),neural cell adhesive molecule(NCAM) and proliferative cell nucleus antigen(PCNA).Results showed that the safe concentration of the extract was from 62.5 mg/L to 8 000 mg/L.Compared with the control group,cell proliferation at the concentration of 125,250 and 500 mg/L increased significantly(P＜0.05).The results of MTT colorimetric test and flow cytometry method showed that the extract could significantly promoted cell proliferation at 125 mg/L(P＜0.01).It suggested that the extract at certain concentration was effective on inducing the proliferation of SCs,and the best concentration was 125 mg/L.At the same time,it could up-regulate the expression of BDNF,NCAM and PCNA of SCs cultured in vitro.
国家科技期刊平台
NSTL
万方数据
维普
