The Staphylococcus aureus was digested by lysostaphin to release type 336 polysaccharides (336PS),gradient concentration ethanol and different enzymes digestion were used for crude extraction,and further purification by gel filtration chromatography was done to obtain pure 336PS.The 336PS were conjugated to BSA by ADH spacing method to prepare a conjugated antigen.And it was purified by gel filtration chromatography and identified by wave scanning from 200 nm to 400 nm.336PS-BSA conjugated antigens subunit vaccine which mixed with oil-adjuvant was used to immunize mice,then antibody detection and immune protection tests were carried out.The results showed that the purity of 336PS was 685.1 g/kg,the peak of the conjugated antigen was ahead of BSA and the highest absorption peak was 212 nm,which moved from 206 nm to 280 nm.336PS-BSA could elicit anti-336PS immune responses,and it could also afford protection for mice challenged by a homeotype strain.
type 336 polysaccharidesStaphylococcus aureusconjugated antigenimmunogenicity
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