首页|中国黄羽鹌鹑消化道四种内分泌细胞的组织分布与形态学观察

中国黄羽鹌鹑消化道四种内分泌细胞的组织分布与形态学观察

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运用免疫组织化学SABC法染色,观察了中国黄羽鹌鹑消化道中4种内分泌细胞的组织分布、密度及形态学特点.结果显示,生长抑素细胞(SS)在腺胃组织中分布密度最高,在小肠内也有分布,而在食管、嗉囊、肌胃、盲肠中未发现有分布.腺胃与其余消化道各部位之间SS细胞的分布存在显著性差异(P<0.05);肠道各部位之间SS细胞的分布不存在显著性差异(P>0.05);SS细胞的分布在雌雄鹌鹑之间不存在显著性差异(P>0.05).五羟色胺细胞在消化道内的分布情况与生长抑素相似,胃泌素阳性细胞与神经肽-Y阳性细胞在消化道各段均无发现.结果表明,中国黄羽鹌鹑内分泌细胞的分布规律和形态特征与消化道各部位的生理功能有密切关系.
Morphological observation and tissue distribution of four kinds of endocrine cells in the digestive tract of Coturnix aponica
The purpose of this paper was to observe tissue distribution,density and morphological characteristics of four kinds of endocrine cells in the digestive tract of Coturnix aponica by using the streptavi din-biotin-peroxidase complex(SABC) immunohistochemical method.In result,the somatostatin secreting cells could obviously be found in proventriculus and duodenum,but not in esophagus,ingluvies,gizzard and caecum.The highest distributive density part was in proventriculus,and the second was in duodenum.Ileum and rectum were in a much lower density.However,the result of statistical analysis showed that there was not significantly difference(P>0.05) among the various parts of the intestine except for the proventriculus which was significantly difference(P<0.05).Besides,the number of somatostatin secreting cells was not significantly(P>0.05) difference between the same parts of female and male,and their shapes were quite the same.The level of 5-hydroxy tryptamine was also the same.Gastrin and neuropeptide could not be found in each part of the digestive tract.In conclusion,the distribution of endocrine cells in the digestive tract of C.aponica is relevant to the function of each part of the digestive tract.

endocrine cellsChinese Yellow quail(Coturnix aponica)immunohistochemical SABC

王磊、何敏

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四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川雅安 625014

内分泌细胞 中国黄羽鹌鹑 免疫组织化学SABC

教育部"长江学者和创新团队发展计划"创新团队项目四川省教育厅科研项目

IRT084810ZB035

2014

中国兽医科学
中国农业科学院兰州兽医研究所

中国兽医科学

CSTPCDCSCD北大核心
影响因子:0.524
ISSN:1673-4696
年,卷(期):2014.44(2)
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