Effects of Marek's disease resistance selection on virus loads in peripheral blood lymphocytes of chickens challenged with Marek's disease virus type 1
Effects of Marek's disease(MD) resistance selection on virus loads in peripheral blood lymphocytes(PBLs) of chickens after the challenge with Marek's disease virus type 1(MDV1) were investigated.Real-time FQ-PCR was used for absolute quantification of the DNA levels of meq gene of PBLs sampled on 7 different time points(on day 4,7,10,14,21,28 and 35 post-infection) from all birds after the challenge of MDV1.The load of MDV1 was detected in PBLs to analyze and compare proliferation of MDV1 in resistant/common or/and HVT-vaccinated/ HVT-unvaccinated birds.In result,the load of MDV1 was significantly higher in chickens of B line (common line,HVT-unvaccinated and MDV1-challenged) but significantly lower in chickens of F line(MD-resistant line,HVT-vaccinated and MDV1-challenged) on day 10(P＜0.05),14(P＜0.05),21(P＜0.01),28(P＜0.05) and 35(P＜0.05) post-infection.There was no significant difference between the other groups.In conclusion,virus loads in chickens can be reduced significantly by resistance selection in coordination with vaccination,which can reduce the harm of MDV1 infection.
Marek's disease virus type 1(MDV1)peripheral blood lymphocytes(PBLs)real-time FQ-PCRresistance selection
国家科技期刊平台
NSTL
万方数据
维普
