Study on anti-aging effect of Orobanche cernua loefling extract on Caenorhabditis elegans
This research aims to observe the role and possible mechanism of Orobanche cernua Loe-fling(OLE)extract on anti-aging.OLE was administered to Caenorhabditis elegans,and relevant physio-logical indicators were measured using ELISA and fluorescent probe method.Additionally,the expres-sion differences of related genes in mutant nematodes were detected by RT-qPCR.The results indicated that the maximum tolerance concentration of N2 wild-type C.elegans to OLE extract was 7.813 g/L.Com-pared with the control group,the OLE with a concentration of 3.91 g/L significantly increased the lifespan of C.elegans under normal,oxidative and heat stress conditions(P＜0.05).The activities of catalase(CAT)and superoxide dismutase(SOD)were significantly increased(P＜O.05),significantly decreased the contents of lipofuscin(LPO),reactive oxygen species(ROS)and malondialdehyde(MDA)(P＜0.05),significantly increased the ratio of daf-16 to skn-1 in C.elegans cells(P＜0.05);Compared with the control group,the lifespan of DA1113,RB1206,CF1903 mutant lines was significantly prolonged after administration(P＜0.05),there was no significant life span extension of CB1370,CF1038,TJ1052,PS3551,QV225 mutant lines after administration(P＜0.05);After administration,the expressions of daf-2,age-1 and akt-1 genes in C.elegans were significantly decreased(P＜0.05)in vivo,the expression levels of daf-16,McL-1,sod-3,ctl-1,hsf-1,hsp-16.2,skn-1 and gcs-1 genes were significantly increased(P＜0.05).The results indicated that the OLE extract extract could improve aging-related indicators of C.elegans by increasing the free radical scavenging ability of C.elegans,increase the ratio of daf-16 and skn-1 entering the nucleus from cytoplasm,boost the antioxidant capacity of C.elegans,and ultimately pro-long the lifespan of them.OLE extract mainly relies on daf-16 and skn-1 to improve the antioxidant stress ability of C.elegans and enhance its anti-aging effect through insulin signaling pathway.
国家科技期刊平台
NSTL
万方数据
维普
