Effects of chlorogenic and caffeic acids on the cryopreservation of semen from Leizhou goats
To improve the quality of frozen-thawed semen,this study investigated the effects of chlorogenic acid(CGA)and caffeic acid(CA)on the cryopreservation of semen from Leizhou goats.100 μmol/L CGA and CA were added to the freezing dilution solution,respectively.The group without addition served as the control,and cryopreservation experiment was conducted.The semen quality was evaluated by assessing frozen-thawed sperm viability,motility,survival rate,plasma membrane integrity,acrosome integrity,and antioxidant indicators such as reactive oxygen species(ROS)level,catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),and mitochondrial membrane potential(MMP).The results showed that the addition of CGA and CA in the cryopreservation diluent significantly improved frozen-thawed semen quality(sperm motility,viability,survival rate,etc.)of Leizhou goats(P＜0.05).The enzyme activities of SOD,GSH-PX,CAT and MMP were ef-fectively increased(P＜0.05),and the sperm ROS level and MDA content were decreased(P＜0.05),so the cryopreser-vation effect of Lezhou goats semen was improved.The research results indicate that the quality and antioxidant properties of Leizhou goats semen are significantly improved after frozen-thawed by adding CGA and CA to the frozen preservation diluent,thereby enhancing the cryopreservation effect of Leizhou goats semen.
chlorogenic acidcaffeic acidLeizhou goatscryopreservation of semenoxidative stress
国家科技期刊平台
NSTL
万方数据
维普
