绿原酸和咖啡酸对雷州山羊精液冷冻保存效果的影响
Effects of chlorogenic and caffeic acids on the cryopreservation of semen from Leizhou goats
苗丰帅 1李靖 1蒋欣怡 1陈宣旭 1于海滨 1姜平 1林紫薇 1赵志辉 1照那木拉1
作者信息
- 1. 广东海洋大学 滨海农业学院,广东 湛江 524088
- 折叠
摘要
为了提高冻-解后精液品质,探讨绿原酸和咖啡酸对雷州山羊精液冷冻保存效果的影响,笔者在冷冻保存稀释液中分别添加100 μmol/L的绿原酸(CGA)和咖啡酸(CA),并以无添加组为对照,实施了冷冻保存实验.通过检测冻-解后精子活率、活力、生存率、质膜完整性、顶体完整性等指标评估精液品质,并检测了活性氧(ROS)水平、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷脱甘肽过氧化物酶(GSH-PX)、氧化产物丙二醛(MDA)、线粒体膜电位(MMP)等抗氧化指标.结果显示,在冷冻保存稀释液中添加CGA和CA可显著(P＜0.05)提高冻-解雷州山羊精液品质(精子活率、活力、生存率等),有效提高SOD、GSH-PX、CAT等酶活性和MMP(P＜0.05),降低了精子ROS水平和MDA的含量(P＜0.05),从而提高了雷州山羊精液冷冻保存效果.结果表明,在冷冻保存稀释液中添加CGA和CA能显著提高冻-解后雷州山羊精液品质和抗氧化性能,进而提高雷州山羊精液冷冻保存效果.
Abstract
To improve the quality of frozen-thawed semen,this study investigated the effects of chlorogenic acid(CGA)and caffeic acid(CA)on the cryopreservation of semen from Leizhou goats.100 μmol/L CGA and CA were added to the freezing dilution solution,respectively.The group without addition served as the control,and cryopreservation experiment was conducted.The semen quality was evaluated by assessing frozen-thawed sperm viability,motility,survival rate,plasma membrane integrity,acrosome integrity,and antioxidant indicators such as reactive oxygen species(ROS)level,catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),and mitochondrial membrane potential(MMP).The results showed that the addition of CGA and CA in the cryopreservation diluent significantly improved frozen-thawed semen quality(sperm motility,viability,survival rate,etc.)of Leizhou goats(P＜0.05).The enzyme activities of SOD,GSH-PX,CAT and MMP were ef-fectively increased(P＜0.05),and the sperm ROS level and MDA content were decreased(P＜0.05),so the cryopreser-vation effect of Lezhou goats semen was improved.The research results indicate that the quality and antioxidant properties of Leizhou goats semen are significantly improved after frozen-thawed by adding CGA and CA to the frozen preservation diluent,thereby enhancing the cryopreservation effect of Leizhou goats semen.
关键词
绿原酸/咖啡酸/雷州山羊/精液冷冻保存/氧化应激Key words
chlorogenic acid/caffeic acid/Leizhou goats/cryopreservation of semen/oxidative stress引用本文复制引用
基金项目
广东省重点领域研发计划项目(2022B0202110002)
广东省乡村振兴战略专项(2022-XPY-00-010)
广东省自然科学基金项目(2021A1515010867)
粤西地区畜禽资源与种质创新重点实验室基金项目(2021A1515010867)
广东海洋大学科研启动基金项目(R17032)
广东海洋大学科研启动基金项目(060302052315)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据