Isolation and identification of bluetongue virus serotype 5 from Culiocides in Yunnan of China
To investigate the prevalence of bluetongue virus(BTV)in Culicoides from Xishuangbanna,Yun-nan province,Culicoides were collected from Menghai and Jinghong in Xishuangbanna from July to August 2022,and bluetongue virus was isolated and identified.Bluetongue virus in Culicoides was detected by qRT-PCR.Among 127 test samples,3 were BTV positive,resulting in a positive rate of 2.36％.These posi-tive samples were inoculated into C6/36 cells for continuous passage,and the third-generation isolat-ed cultures exhibited a significant cytopathic effect(CPE).The isolated cultures were confirmed to con-tain BTV by qRT-PCR,and the isolates were all BTV-5 based on the sequence analysis of L2 gene.These L2 gene sequences of the 3 isolates shared more than 93％similarity with the reference BTV-5 strain(AJ585126.1)and over 98％similarity with the BTV-5 strain(KT945045.1)isolated from Shizong,Yunnan province.The simi-larity of the L3 gene sequence from the 3 isolates with BTV-4(JX560415.1)isolated from Mangshi of Yunnan province was more than 96.8％.These results underscore the prevalence of BTV-5 in Culicoides in Xishuangbanna,Yunnan province.This study provided data for the study of bluetongue virus vector and the prevention and control of bluetongue disease.
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