首页|PRRSV BZ4-19株的分离鉴定及其全基因序列分析

PRRSV BZ4-19株的分离鉴定及其全基因序列分析

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  • 国家科技期刊平台
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为了解山东滨州某猪场PRRSV的流行及变异情况,将该猪场采集的疑似PRRSV阳性病料进行RT-PCR检测,检测出的阳性样品用Marc-145细胞进行分离,设计了 9对扩增引物用于分离株(BZ4-19)的全基因扩增,将扩增出的全基因序列进行核苷酸及其推导的氨基酸序列分析.最终成功在Marc-145细胞上分离到1株RespPRRS MLV-like毒株,全基因扩增结果显示其全长为15 412 bp(不含polyA尾);核苷酸同源性分析说明,其与美洲经典株VR2332及RespPRRS MLV(疫苗株)同源性最高,分别为99.4％和99.8％.将BZ4-19分离株与VR2332、RespPRRS MLV的氨基酸序列进行比较分析揭示,VR2332与RespPRRS MLV共有30处氨基酸差异,在这30处差异中,分离株与VR2332有7处一致,与RespPRRS MLV有23处一致.在鉴别VR2332与RespPRRS MLV的9处可能与毒力相关的氨基酸位点时,分离株有7处与VR2332相同,由此推测本研究分离到的毒株属于疫苗株RespPRRS MLV返强毒株,我国具有上述分子变化的PRRSV的流行应被关注.
Isolation,identification and complete gene sequence analysis of PRRSV BZ4-19 strain
To understand the prevalence and variation of PRRSV in a pig farm in Binzhou,Shandong Province,China,suspected PRRSV-positive material collected from this farm was subjected to RT-PCR.The detected positive samples were isolated using Marc-145 cells,then nine pairs of amplification primers were designed for whole gene amplification of the isolate(named BZ4-19),and the amplified whole gene sequence was analyzed for nucleotides and their deduced amino acid sequences.The results showed that a RespPRRS MLV-like strain was successfully isolated from Marc-145 cells,and the whole gene am-plification showed that its full length was 15 412 bp(without polyA).Nucleotide homology analysis showed that it had the highest homology with the American classical strain VR2332 and RespPRRS MLV(vaccine strain),with 99.4％and 99.8％.Amino acid sequences of BZ4-19 isolates were analyzed in comparison with those of VR2332,RespPRRS MLV,the results showed that VR2332 and RespPRRS MLV had a total of 30 amino acid differences.Of these 30 differences,the isolate was consistent with VR2332 in 7 instances and with RespPRRS MLV in 23 instances.In the identification of nine amino acid sites that may be related to virulence between VR2332 and RespPRRS MLV,seven of the isolates were identical to VR2332.It is hy-pothesized that the strain isolated in this study belongs to the vaccine strain RespPRRS MLV returned strong strain,and the prevalence of PRRSV with the above molecular changes in China should be con-cerned.

porcine reproductive and respiratory syndrome virus(PRRSV)classical American strainRespPRRS MLV strainvirus isolation and characterizationepidemiology

李思琪、刘莹、刘濮毓、任晓祥、李岩岩、王文钊、左玉柱、范京惠

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河北农业大学动物医学院,河北保定 071001

河北省兽医生物技术创新中心,河北保定 071001

猪繁殖与呼吸障碍综合征病毒(PRRSV) 经典美洲株 RespPRRS MLV株 病毒分离鉴定 流行病学

河北省农业产业技术体系生猪创新团队项目河北省农业产业技术体系生猪创新团队项目石家庄市科技计划项目

HBCT2024220201HBCT2024220401229500232N

2024

10.16656/j.issn.1673-4696.2024.0012

中国兽医科学
中国农业科学院兰州兽医研究所

中国兽医科学

CSTPCD北大核心
影响因子：0.524
ISSN：1673-4696
年,卷(期)：2024.54(2)
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