摘要
牛呼吸道合胞体病是牛呼吸道合胞体病毒(BRSV)引起的呼吸道疾病,为一种急性、热性、高度传染性疾病.BRSV的感染率高,死亡率低,常继发性细菌感染.我国BRSV血清阳性率为41.2％～94.4％,鉴于国内无BRSV有效的疫苗及药物,为了控制BRSV的传播和高效诊断,便于各类兽医机构选择合适的检测方法,本文就现有的BRSV的分离鉴定技术、RT-PCR、荧光定量RT-PCR、纳米RT-PCR、逆转录恒温隔绝式RT-PCR(iiRT-PCR)、巢式PCR、环介导等温扩增技术(LAMP)、基因组测序技术、免疫组织化学(IHC)、直接免疫荧光试验(d-FAT)、病毒中和试验(VNT)、间接ELISA、双抗体夹心ELISA(DAS-ELISA)等检测技术予以综述.
Abstract
Bovine respiratory syncytiosis is a respiratory disease caused by bovine respiratory syncytial virus(BRSV),which is an acute,febrile and highly infectious disease;BRSV has high infection rate,low mortali-ty,and often secondary bacterial infection.The seropositive rate of BRSV in China is 41.2％-94.4％.In view of the lack of effective vaccines and drugs for BRSV in China,in order to control the transmission and efficient diag-nosis of BRSV,and to facilitate various veterinary institutions to select appropriate detection methods,in this paper the isolation and identification of BRSV,RT-PCR,fluorescent quantitative RT-PCR,nano RT-PCR,re-verse transcription isothermal isolation RT-PCR(iiRT-PCR),nested PCR,loop mediated isothermal amplifiica-tion(LAMP),genome sequencing technology,immunohistochemistry(IHC),direct immunofluorescence(D-FAT),virus neutralization test(VNT),indirect ELISA,double antibody sandwich ELISA(DAS-ELISA)were reviewed and compared.
基金项目
中央引导地方科技发展资金项目(236Z6604G)
河北省现代农业产业技术体系肉牛产业创新团队疫病防控与减抗岗位项目(HBCT2023190201)
河北省重点研发计划乡村振兴技术创新专项(22326612D)
国家科技期刊平台
NSTL
万方数据