Research progress in detection methods for bovine respiratory syncytial virus
Bovine respiratory syncytiosis is a respiratory disease caused by bovine respiratory syncytial virus(BRSV),which is an acute,febrile and highly infectious disease;BRSV has high infection rate,low mortali-ty,and often secondary bacterial infection.The seropositive rate of BRSV in China is 41.2％-94.4％.In view of the lack of effective vaccines and drugs for BRSV in China,in order to control the transmission and efficient diag-nosis of BRSV,and to facilitate various veterinary institutions to select appropriate detection methods,in this paper the isolation and identification of BRSV,RT-PCR,fluorescent quantitative RT-PCR,nano RT-PCR,re-verse transcription isothermal isolation RT-PCR(iiRT-PCR),nested PCR,loop mediated isothermal amplifiica-tion(LAMP),genome sequencing technology,immunohistochemistry(IHC),direct immunofluorescence(D-FAT),virus neutralization test(VNT),indirect ELISA,double antibody sandwich ELISA(DAS-ELISA)were reviewed and compared.
bovine respiratory syncytium virusisolation and identificationtest method
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