细胞自噬相关蛋白ATG9A对口蹄疫病毒侵入的影响
Effect of cellular autophagy-related protein ATG9A on invasion of foot-and-mouth disease virus
邓欣雨 1袁红 1王涛 1宫晓华 1赵俊芳 1包慧芳 1李平花 1孙普 1付元芳 1李坤 1刘在新 1卢曾军 1白兴文1
作者信息
- 1. 中国农业科学院兰州兽医研究所兰州大学动物医学与生物安全学院动物疫病防控全国重点实验室,甘肃兰州 730000
- 折叠
摘要
为揭示细胞自噬相关蛋白9A(ATG9A)对口蹄疫病毒(FMDV)复制的影响,利用IFA、RT-qPCR和Western-blot等方法检测FMDV与ATG9A的共定位情况以及病毒感染对ATG9A的影响;通过构建ATG9A重组表达质粒和合成小干扰RNA,检测过表达及敲降ATG9A对FMDV吸附、内化、基因组复制、蛋白翻译、子代病毒粒子产生的影响.结果显示,FMDV感染后与ATG9A共定位且抑制ATG9A的表达;ATG9A不影响FMDV的吸附,但极显著地抑制FMDV的内化过程;同时,过表达(或干扰)ATG9A能够在RNA与蛋白水平显著降低(或提高)FMDV的增殖和子代病毒粒子产生的能力.结论,ATG9A抑制FMDV的复制,为深入认识FMDV在细胞内的复制周期提供了线索.
Abstract
In order to reveal the effect of cellular autophagy-related protein 9A(ATG9A)on foot-and-mouth disease virus(FMDV)replication,the co-localization of FMDV and ATG9A as well as the im-pact on ATG9A expression during viral infection were detected by indirect immunofluorescence assay,reverse transcription quantitative real-time PCR and Western-blot methods.The recombinant plasmid for expression of ATG9A and small interfering RNA targeting ATG9A were constructed and employed to esti-mate the influence for adsorption,internalization,genomic replication,polyprotein translation,and progeny production of FMDV,respectively.The results showed that the FMDV was co-localized with ATG9A and the expression of ATG9A was down-regulated after FMDV infection.ATG9A could not affect virus ad-sorption,but significantly inhibited FMDV internalization.Furthermore,overexpress ion(or knock-down)of ATG9A dramatically reduced(or increased)FMDV proliferation and the production of progeny virions.In conclusion,ATG9A might be deleterious to FMDV replication,which would provide insights into the replication cycle of FMDV in susceptible cells.
关键词
自噬相关蛋白9A/口蹄疫病毒/内化/复制Key words
autophagy-related protein 9A/foot-and-mouth disease virus/internalization/repli-cation引用本文复制引用
基金项目
国家重点研发计划(2021YFD1800300)
国家自然科学基金(31772763)
甘肃省科技重大专项(22ZD6NA012)
甘肃省科技重大专项(22ZD6NA001)
甘肃省科技重大专项(21ZD3NA001)
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03)
出版年
2024
国家科技期刊平台
NSTL
万方数据