首页|细胞自噬相关蛋白ATG9A对口蹄疫病毒侵入的影响

细胞自噬相关蛋白ATG9A对口蹄疫病毒侵入的影响

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
为揭示细胞自噬相关蛋白9A(ATG9A)对口蹄疫病毒(FMDV)复制的影响,利用IFA、RT-qPCR和Western-blot等方法检测FMDV与ATG9A的共定位情况以及病毒感染对ATG9A的影响;通过构建ATG9A重组表达质粒和合成小干扰RNA,检测过表达及敲降ATG9A对FMDV吸附、内化、基因组复制、蛋白翻译、子代病毒粒子产生的影响.结果显示,FMDV感染后与ATG9A共定位且抑制ATG9A的表达;ATG9A不影响FMDV的吸附,但极显著地抑制FMDV的内化过程;同时,过表达(或干扰)ATG9A能够在RNA与蛋白水平显著降低(或提高)FMDV的增殖和子代病毒粒子产生的能力.结论,ATG9A抑制FMDV的复制,为深入认识FMDV在细胞内的复制周期提供了线索.
Effect of cellular autophagy-related protein ATG9A on invasion of foot-and-mouth disease virus
In order to reveal the effect of cellular autophagy-related protein 9A(ATG9A)on foot-and-mouth disease virus(FMDV)replication,the co-localization of FMDV and ATG9A as well as the im-pact on ATG9A expression during viral infection were detected by indirect immunofluorescence assay,reverse transcription quantitative real-time PCR and Western-blot methods.The recombinant plasmid for expression of ATG9A and small interfering RNA targeting ATG9A were constructed and employed to esti-mate the influence for adsorption,internalization,genomic replication,polyprotein translation,and progeny production of FMDV,respectively.The results showed that the FMDV was co-localized with ATG9A and the expression of ATG9A was down-regulated after FMDV infection.ATG9A could not affect virus ad-sorption,but significantly inhibited FMDV internalization.Furthermore,overexpress ion(or knock-down)of ATG9A dramatically reduced(or increased)FMDV proliferation and the production of progeny virions.In conclusion,ATG9A might be deleterious to FMDV replication,which would provide insights into the replication cycle of FMDV in susceptible cells.

autophagy-related protein 9Afoot-and-mouth disease virusinternalizationrepli-cation

邓欣雨、袁红、王涛、宫晓华、赵俊芳、包慧芳、李平花、孙普、付元芳、李坤、刘在新、卢曾军、白兴文

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中国农业科学院兰州兽医研究所兰州大学动物医学与生物安全学院动物疫病防控全国重点实验室,甘肃兰州 730000

自噬相关蛋白9A 口蹄疫病毒 内化 复制

国家重点研发计划国家自然科学基金甘肃省科技重大专项甘肃省科技重大专项甘肃省科技重大专项国家生猪技术创新中心先导科技项目

2021YFD18003003177276322ZD6NA01222ZD6NA00121ZD3NA001NCTIP-XD/C03

2024

10.16656/j.issn.1673-4696.2024.0049

中国兽医科学
中国农业科学院兰州兽医研究所

中国兽医科学

CSTPCD北大核心
影响因子：0.524
ISSN：1673-4696
年,卷(期)：2024.54(3)
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