Correlation between the expression of IL-4 and IL-5 and central nervous system injury caused by HEV infection in tree shrews
Hepatitis E virus(HEV)can cause extrahepatic injury of the nervous system,but its mech-anism is still unclear.The experimental infection model of HEV tree shrew was established by in-traperitoneal injection.RT-PCR was used to detect HEV negative strand RNA in feces,liver,brain and other tissues,real-time fluorescence quantitative PCR was used to detect the growth and decline of virus in brain,feces,small intestine and liver of infected tree shrews,the levels of AST and ALT were determined,and histopathology was performed to observe the damage of each tissue.And the expression levels of inflammatory cytokines IL-4 and IL-5 in different time periods of infection.The results showed that the virus negative strand RNA replication could be detected in all tissues on the 7 days post infection(DPI).AST and ALT enzymes were significantly increased compared with control group.Histopathological observation revealed that the liver and other tissues were damaged to different de-grees,meninges were dilated and congested,glial cells infiltrated,cerebellum showed a large number of vacuole-like structures and white matter congestion,and various types of neurons in the spinal cord had different shapes and sizes,with more neurons becoming denaturated and necrosis,nucleus disinte-gration and contraction,and glial cell hyperplasia.IL-4 and IL-5 mRNA transcription levels increased significantly in the early and middle stages of infection.The results show that HEV can break through the blood-brain barrier and enter the parenchymal tissues such as brain and spinal cord,and cause pathological injury of brain and spinal cord.IL-4 and IL-5 increase in different tissues in the early stage of inf ect ion,which is related to the injury of brain and spinal cord tissues caused by HEV infection.
hepatitis E virustree shrewcentral nervous system injuryIL-4IL-5
国家科技期刊平台
NSTL
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