袖状胃切除术对大鼠糖代谢及脏器Ghrelin和IGF-I的影响
Effect of sleeve gastrectomy on glucose-metabolism and Ghrelin and IGF-Ⅰ in rats
顾伟芳 1蔡鹏 1朱江 1刘璐 1倪艳 1马思远 1段兴勋 1高仕文 1赵红琼1
作者信息
- 1. 新疆农业大学动物医学学院,新疆乌鲁木齐 830052
- 折叠
摘要
袖状胃切除术,又称缩胃手术,主要用于减重和2型糖尿病的治疗.为了探究袖状胃切除术对大鼠糖代谢及生长调控相关激素Ghrelin、IGF-Ⅰ的影响.本研究将试验组SD大鼠行袖状胃切除术,对照组行假手术.术后第7天,检测血浆中Ghrelin、IGF-Ⅰ、葡萄糖含量及脏器组织样本中的IGF-Ⅰ、IGFBP-3、GHS-R及GH-R的mRNA表达量.试验组术后血浆中Ghrelin、IGF-Ⅰ水平极显著低于对照组(P＜0.01),肝脏、肾脏及肺脏的IGF-Ⅰ和GH-R的mRNA表达量均极显著低于对照组(P＜0.01),肝脏的IGFBP-3和肾脏的GHS-R的mRNA也极显著低于对照组(P＜0.01).此外,试验组术后血糖水平极显著下降(P＜0.01),胰岛素水平也极显著低于对照组(P＜0.01).本研究结果表明袖状胃切除后大鼠Ghrelin、IGF-Ⅰ等激素水平显著下降,同时伴随着血糖显著下降,提示袖状胃切除导致的激素水平变化可能介导调控机体糖代谢.
Abstract
Sleeve gastrectomy,also known as gastric reduction surgery,is mainly used for weight loss and the treatment of type 2 diabetes.This study aimed to investigate the effect of sleeve gastrectomy on glucose-metabolism and growth-regulating hormones Ghrelin and IGF-Ⅰ in rats.In this study,the SD rats in the experimental group underwent sleeve gastrectomy,and the control group underwent sham surgery.On the 7th postoperative day,the plasma contents of ghrelin,IGF-Ⅰ and glucose and the mRNA expression level of IGF-Ⅰ,IGFBP-3,GHS-R and GH-R were measured in organ tissue samples.The levels of ghrelin and IGF-Ⅰ in plasma of experimental group after surgery were significantly lower than those of control group(P＜0.01),and the expression levels of IGF-Ⅰ and GH-R mRNA in the liver,kidney and lung were significantly lower than those of control group(P＜0.01).The mRNA levels of IGFBP-3 in liver andGHS-R in kidney were also significantly lower than those in control group(P＜0.01).In addition,the post-operative blood glucose level of the experimental group decreased significantly(P＜0.01),and the insulin level was significantly lower than that of control group(P＜0.01).The results of this study showed Ghrelin,IGF-Ⅰ and other hormones decreased significantly in rats after sleeve gastrectomy,accompanied by a significant decrease in blood glucose,suggesting that the changes in hormone levels caused by sleeve gastrectomy may affect mediate the regulation of glucose metabolism.
关键词
大鼠/袖状胃切除术/生长激素释放肽/胰岛素样生长因子-Ⅰ/血糖Key words
rat/sleeve gastrectomy/growth hormone releasing peptide/insulin-like growth factor-Ⅰ/blood glucose引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据