首页|2021-2022年华东地区猪繁殖与呼吸综合征病毒的分子流行病学调查

2021-2022年华东地区猪繁殖与呼吸综合征病毒的分子流行病学调查

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为初步了解我国华东地区猪繁殖与呼吸综合征病毒(PRRSV)的流行特点,2021-2022年采集华东地区480份临床疑似PRRSV样品,采用RT-PCR方法检测是否存在PRRSV,统计显示阳性为175份,阳性检出率为38.89%.对来自不同猪场的24个阳性样品的病毒ORF5基因测序,所有检测的毒株均属美洲型,共可以分为4个亚型,其中11个毒株属于高致病性PRRSV(HP-PRRSV)(Sublineage 8.7);6个毒株属于类NADC30毒株亚型(Sublineage 1.8);4个毒株属于类NADC34亚型(Sublineage 1.5).病毒GP5蛋白基因测序结果显示,流行毒株GP5蛋白aa3-39及aa185-200两个高变区出现变异.该研究表明我国该地区PRRSV流行毒株存在遗传多样性,基因型复杂,需要引起我们的重视.
Molecular epidemiological survey of porcine reproductive and respiratory syndrome virus in East China from 2021 to 2022
In order to preliminarily study the epidemic characteristics of porcine reproductive and respiratory syndrome virus(PRRSV)in East China,480 clinically suspected PRRSV-positive samples were collected in East China from 2021 to 2022 for PRRSV detection by RT-PCR.Of these samples,175 were PRRSV-positive with a positive rate of 38.89%.The ORF5 genes of 24 positive samples from different pig farms were sequenced and shown to be the American type 2,divided into 4 subtypes.Among them,11 strains belonged to the HP-PRRSV(Sublineage 8.7),6 strains belonged to the PRRSV NADC30-like strain(Sublineage 1.8),and 4 strains belonged to the PRRSV NADC34-like strain(Sublineage 1.5).The PRRSV GP5 sequencing results showed that mutations occurred in the two hypervariable regions aa3-39 and aa185-200 of the epidemic strains.This study shows that the circulating PRRSV strains in East China have a high genetic diversity with complex genotypes,which calls for our special attention.

porcine reproductive and respiratory syndrome virus(PRRSV)ORF5NADC34NADC30genetic variation

张杰、张路捷、周昊然、刘星、白娟、姜平

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南京农业大学动物医学院农业部动物细菌学重点实验室,江苏南京 210095

猪繁殖与呼吸综合征病毒 ORF5 NADC34 NADC30 遗传变异

江苏省农业科技自主创新项目国家生猪产业技术体系建设专项国家自然科学基金

CX221011CARS-3632230103

2024

中国兽医科学
中国农业科学院兰州兽医研究所

中国兽医科学

CSTPCD北大核心
影响因子:0.524
ISSN:1673-4696
年,卷(期):2024.54(4)
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