Protective effect of puerarin on mitochondrial damage caused by cadmium in the cerebral cortices of rat
To study the protective effect of puerarin on cadmium-induced mitochondrial damage,twenty-four SD rats were randomly divided into control group(Con),cadmium group(Cd),puerarin group(Pur),cadmium and puerarin co-treated group(Pur+Cd).The Pur group and Pur+Cd treated group underwent gavage of puerarin dissolved in sodium carboxymethyl cellulose at a dose of 200 mg/kg body weight,while the Con group and Cd treated group were given the same dose of sodium carboxymethyl cellulose by gavage every day for 7 weeks.The Cd group and Pur+Cd treated group received drinking water containing 75 mg/L Cd plus the gavage from the 4th week and lasted for 4 weeks.After the experimental trail comple-tion,the rats were dissected and collected the cerebral cortices.The cadmium contents were detected by flame atomic absorption spectrometry.ATP level was detected by luminometer.The ratio of NAD+/NADH,the activity of MnSOD and the level of H2O2 were detected by colorimetry.The transcriptional levels of mito-chondrial oxidative stress-related genes were detected by real-time quantitative polymerase chain re-action.The results showed that compared with the Con group,the cadmium contents,the level of H2O2 and the transcription levels of mitochondrial oxidative stress-related genes significantly increased(P＜0.05),while ATP level,the ratio of NAD+/NADH and the activity of MnSOD significantly decreased(P＜0.05)in cerebral cortices of rats in Cd group.Compared with the Cd group,the cadmium contents,the level of H2O2 and the transcription levels of mitochondrial oxidative stress-related genes signifi-cantly decreased(P＜0.05),while the activity of MnSOD significantly increased(P＜0.05),ATP level and the ratio of NAD+/NADH increased without significant differences(P＞0.05)in cerebral cortices of rats in Cd+Pur group.The results indicate that puerarin reduces cadmium contents in the cerebral cortices of cadmium-exposed rats.Puerarin can alleviate the mitochondrial damage caused by cadmium in the rat cerebral cortices by alleviating mitochondrial dysfunction and mitochondrial oxidative stress.
cadmiumpuerarincerebral cortices of ratsmitochondrial damage
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