首页|宿主OAS2蛋白抑制非洲猪瘟病毒体外复制的研究

宿主OAS2蛋白抑制非洲猪瘟病毒体外复制的研究

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  • 国家科技期刊平台
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为了探究宿主蛋白 2′,5′-寡腺苷酸合成酶 2(OAS2)与非洲猪瘟病毒(ASFV)复制之间的关系,本研究通过蛋白免疫印迹(Western-blot)和实时荧光定量PCR(RT-qPCR)分析了ASFV 感染对宿主OAS2的调控作用;构建并合成OAS2 真核表达质粒以及siRNA干扰序列,通过RT-qPCR、Western-blot方法探究在MA-104 细胞中外源过表达OAS2 及在PAMs细胞中下调OAS2 表达对ASFV 体外复制的影响.结果显示,ASFV感染PAMs细胞后,OAS2 的蛋白水平和转录水平皆上调,并且外源过表达OAS2 显著抑制了ASFV的复制,下调OAS2表达促进了ASFV的复制.本研究通过对宿主蛋白OAS2进行过表达和敲低,证实了OAS2可以抑制ASFV体外复制,该结果为进一步研究宿主对ASFV的抗病毒免疫研究提供了理论基础.
Inhibition of African swine fever virus replication by the host OAS2 protein in vitro
In order to investigate the relationship between host protein 2′,5′-oligoadenylate synthetase 2(OAS2)and the replication of African swine fever virus(ASFV),the present study was conducted to analyze the regulation of OAS2 in the host by ASFV infection by protein immunoblotting(Western-blot)and real-time fluorescence quantitative PCR(RT-qPCR).OAS2 was constructed and synthesized as a eukaryotic expression plasmid and siRNA interfering sequences,and its role was investigated in MA-104 by RT-qPCR and Western-blot.In this study,we analyzed the regulatory effect of ASFV infection on host OAS2 by protein immunoblotting(Western-blot)and real-time fluorescence quantitative PCR(RT-qPCR).We constructed and synthesized the eukaryotic expression plasmid of OAS2 and the siRNA interfering sequences,and we investigated the effects of ectopic overexpression of OAS2 in MA-104 cells and the down-regulation of OAS2 in PAMs on the in vitro replication of ASFV by RT-qPCR and Western-blot.The results showed that the protein and transcript levels of OAS2 were up-regulated after ASFV infection in PAMs cells.Moreover,exogenous overexpression of OAS2 significantly inhibited ASFV replication,and down-regulation of OAS2 expression promoted ASFV replication.In this study,we confirmed that OAS2 could inhibit ASFV replication in vitro by overexpressing and knocking down the host protein OAS2,and the results provide a theoretical basis for further research on host antiviral immunity against ASFV.

African swine fever virusOAS2innate immunityinhibitory

赵思越、史喜绢、杨行、张大俊、赵登率、陈国辉、陈玲玲、闫文倩、别鑫恬、赵美玉、李平、郑海学、张克山、郜原

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甘肃农业大学 生命科学技术学院,甘肃 兰州 730070

中国农业科学院 兰州兽医研究所 兰州大学动物医学与生物安全学院 动物疫病防控全国重点实验室,甘肃 兰州 730000

非洲猪瘟病毒 OAS2 天然免疫 抑制

国家重点研发计划国家生猪产业技术体系建设项目中央高校基本科研业务费专项国家生猪技术创新中心先导科技项目甘肃省科技重大专项广东省农业科技创新十大主攻方向"揭榜挂帅"项目(十四五)中国农科院科技创新工程项目中国农科院基础科学中心重大任务项目

2021YFD1801300CARS-35NCTIP-XD/C0322ZD6NA0012022SDZG02CAAS-ASTIP-2022-LVRICAAS-CSLPDC-202302

2024

10.16656/j.issn.1673-4696.2024.0085

中国兽医科学
中国农业科学院兰州兽医研究所

中国兽医科学

CSTPCD北大核心
影响因子：0.524
ISSN：1673-4696
年,卷(期)：2024.54(5)
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