Establishment and application of double RT-PCR detection method for goose astrovirus and novel duck reovirus
In this study,specific primers were designed based on the conserved sequence of goose astrovirus(GAstV)ORFlb gene and novel duck reovirus(NDRV) σ C gene,and a dual RT-PCR method of GAstV and NDRV was established.This method has high specificity to amplify fragments of 903 bp(GAstV)and 226 bp(NDRV).The minimum sensitivity of this method was 1.0 × 103 copies/μL.In 320 samples randomly taken from goose farms of different scales in the central,northwestern,and southern regions of Shandong,the positive rate of GAstV was 21.56％(69/320),the positive rate of NDRV was 3.44％(11/320),and the posi-tive rate of mixed infection was 2.81％(9/320).The results of the single RT-PCR were consistent with the dual method.This provides a reliable basis for the rapid diagnosis of GAstV and NDRV.
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