首页|邓氏巴贝斯虫血清学诊断抗原的筛选

邓氏巴贝斯虫血清学诊断抗原的筛选

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  • 国家科技期刊平台
  • NETL
  • NSTL
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为了鉴定用于邓氏巴贝斯虫检测的潜在抗原,本研究通过蛋白质组学筛选邓氏巴贝斯虫感染后释放到宿主血液中的分泌性抗原,结合生物信息学、分子生物学技术和血清学反应分析并验证其作为诊断抗原的潜力.结果表明,蛋白质组学共鉴定到34种分泌性抗原,生物信息学分析显示,其中8种蛋白在近源物种中没有同源蛋白,为邓氏巴贝斯虫物种特异性抗原,6种蛋白能在真核细胞中表达,2种蛋白(ExoAg-1200和ExoAg-2451)可与邓氏巴贝斯虫阳性血清发生特异性反应,而与健康金黄地鼠血清无反应.上述结果表明,本研究获得了两种邓氏巴贝斯虫物种特异性抗原,为今后建立其血清学检测方法提供了生物材料.
Screening of serological diagnostic antigens for Babesia duncani infection
In order to identify potential diagnostic antigens for detecting Babesia duncani infection,exoantigens(ExoAgs)secreted into host sera were screened by quantitative proteomics,and bioinformatics analysis,molecular biology techniques,and serological reactions were used to analyze and validate their potential as diagnostic antigens.The results showed that a total of 34 ExoAgs were identified using proteomics techniques.Bioinformatics analysis revealed that 8 ExoAgs did not have homologous proteins in Apicomplexan parasites,thus,these 8 ExoAgs were regarded as B.duncani species-specific antigens.Further analysis found that 6 ExoAgs were successfully expressed in eukaryotic cells,and 2 proteins(ExoAg-1200 and ExoAg-2451)could react specifically with B.duncani positive sera,but no reaction with healthy hamster sera.These results indicate that 2 B.duncani species-specific antigens were obtained in this study,and our research may provide biomaterials for the establishment of B.duncani serological detection methods in the future.

Babesia duncanibabesiosisexoantigensproteomicsdiagnostic antigen

年月丽、王锦明、张尚弟、张卫军、关贵全、殷宏、尤崇革

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兰州大学第二医院检验医学中心,甘肃兰州 730030

中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室甘肃省动物寄生虫病重点实验室,甘肃兰州 730046

江苏高校动物重要疫病与人兽共患病防控协同创新中心,江苏扬州 225009

邓氏巴贝斯虫 巴贝斯虫病 分泌性抗原 蛋白质组学 诊断抗原

国家重点研发计划(十四五)国家自然科学基金国家寄生虫资源库项目国家肉牛牦牛产业技术体系建设项目甘肃省自然科学基金甘肃省基础研究创新群体项目甘肃省科技厅特派团项目甘肃省自然科学基金全国实验室重大成果培育项目农业科技创新工程项目

2022YFD180020031972701NPRC-2019-194-30CARS-3722JR5RA03122JR5RA02422CX8NA01123JRRA1632SKLADCP2023HP04

2024

10.16656/j.issn.1673-4696.2024.0092

中国兽医科学
中国农业科学院兰州兽医研究所

中国兽医科学

CSTPCD北大核心
影响因子：0.524
ISSN：1673-4696
年,卷(期)：2024.54(6)
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