首页|长角血蜱Septin-3的原核表达及其免疫分析

长角血蜱Septin-3的原核表达及其免疫分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
为探究长角血蜱Septin-3蛋白的结构、生物学功能和免疫特性,通过基因克隆和原核蛋白表达解析Septin-3蛋白的分子结构,利用制备多克隆抗体、ELISA检测抗体效价和Western-blot检测分析Septin-3蛋白的免疫特性,结合动物感染试验验证Septin-3蛋白的功能.结果显示,Septin-3基因的目的片段大小为1 017 bp,可编码约338个氨基酸残基,表达38 ku大小的蛋白,抗体效价较高,为1∶256 000.新西兰大白兔攻蜱试验结果显示,与对照组相比,经重组Septin-3蛋白免疫后长角血蜱的饱血时间增加了 1.34 d,饱血后重量下降了 20.03～24.75 mg,饱血率下降了 11.04％.研究结果提示,重组Septin-3蛋白具有良好的免疫原性和反应原性,对长角血蜱的生长发育有一定的抑制作用.
Prokaryotic expression and immune analysis of Septin-3 in Haemaphysalis longicornis(Acari:Ixodidae)
To investigate the structure,biological function and immunological properties of Septin-3 protein of Haemaphysalis longicornis,molecular structure of Septin-3 protein was resolved by gene cloning and prokaryotic protein expression,the immune properties were analyzed using the preparation of polyclonal antibodies,ELISA for antibody potency and Western-blot detection,and Septin-3 protein function was validated in conjunction with animal infection experiment.The results showed that the target fragment size of Septin-3 gene was 1 017 bp,and the Septin-3 gene can encode about 338 amino acid residues,and it can express a protein of 38 ku,the antibody potency of the Septin-3 protein was as high as 1∶256 000.The study conducted tick attack experiments on New Zealand white rabbits,compared with the control group,the results showed that H.longicornis,its days of saturation increased by 1.34 d,its weight after saturation decreased by 20.03-24.75 mg,and its saturation rate decreased by 11.04％.The research results suggest that the recombinant Septin-3 protein has excellent immunogenicity and reactivity,and has a certain inhibitory effect on the growth and development of H.longicornis.

Haemaphysalis longicornisSeptin-3 geneprokaryotic expressionantibodyimmune analysis

车丽妍、王彦龙、张振杰、刘光远、关贵全、殷宏、任巧云、赵爱云、王国辉、张文霞、罗金、齐萌

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塔里木大学动物科学与技术学院,新疆阿拉尔 843300

中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室,甘肃兰州 730046

长角血蜱 Septin-3基因 原核表达 抗体 免疫分析

国家寄生虫资源中心项目中国农科院科技创新工程项目(ASTIP)甘肃省基础研究创新群体项目国家肉牛牦牛产业技术体系建设项目家畜疫病病原生物学国家重点实验室开放基金

2019-194-3022JR5RA024CARS-37SKLVEB2021KFKT012

2024

10.16656/j.issn.1673-4696.2024.0108

中国兽医科学
中国农业科学院兰州兽医研究所

中国兽医科学

CSTPCD北大核心
影响因子：0.524
ISSN：1673-4696
年,卷(期)：2024.54(6)
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