Proliferative properties of maedi-visna virus in four cell types of sheep
In order to understand the tropism of Maedi-Visna virus(MVV)infection in different tissues of sheep and its proliferation in different cells,the MVV A2 strain was inoculated into OAR-L1,SCP,STCs,and FLK-BLV.The cytopathic features of cells and the relative expression level of the MVV in-fection-causing CA protein were observed through indirect immunofluorescence assay and Western-blot assay at different time points to determine the viral proliferation in cells.The results showed that SCP and OAR-L1 cells exhibited sustained proliferation in the inoculation group.SCP cells showed cyto-pathic changes such as syncytia,and the degree of cell death increased with the duration of viral in-fection,OAR-L1 cells showed significant cell death with prolonged viral infection,but typical cyto-pathic changes such as multinucleated giant cells were not observed.The control and blank groups of cells remained in good condition without significant differences.FLK-BLV and STCs cells in the inocu-lation group,control group,and blank group did not show significant changes.Indirect immunofluores-cence results showed green fluorescence in SCP and OAR-L1 cells in the inoculation group,while FLK-BLV and STCs cells in the inoculation group did not show green fluorescence compared to the control and blank groups.The green fluorescence area gradually expanded with the prolongation of viral infection in SCP and OAR-L1 cells.The green fluorescence area reached over 90％after 9 days of inoculation in SCP cells,which was considered the optimal infection time.The green fluorescence area reached over 90％after 12 days of inoculation in OAR-L1 cells,which was considered the optimal infection time.West-ern-blot results showed a clear band at 25 ku,and the relative expression level of CA protein gradually increased with the prolongation of inoculation time,showing extremely significant differences com-pared to the control and blank groups(P＜0.000 1)according to ImageJ grayscale analysis.In conclu-sion,this study found that the expression level of viral protein gradually increased with the duration of infection in SCP and OAR-L1 cells after infection with MVV A2 strain.It has been observed that MVV can sustain infection in these two types of cells,and one cell line capable of continuous infection and production of lentiviruses has been identified.
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