CpG和poly I∶C增强口蹄疫病毒样颗粒疫苗免疫效果的研究
Enhancing immune effect of foot-and-mouth disease virus-like particles vaccine with CpG and poly I∶C
任丽华 1罗新 2董虎 2谭书桢 2郭慧琛 2孙世琪 2魏彦明 1滕志东2
作者信息
- 1. 甘肃农业大学动物医学院,甘肃兰州 730070
- 2. 中国农业科学院 兰州兽医研究所 兰州大学动物医学与生物安全学院 动物疫病防控全国重点实验室,甘肃兰州 730046
- 折叠
摘要
本研究旨在评估CpG和polyI∶C对口蹄疫(FMD)病毒样颗粒(VLPs)疫苗的免疫增强效果.以纯化的O型FMD VLPs抗原为基础,使用VLPs-CpG和VLPs-polyI∶C刺激小鼠骨髓源性树突状细胞(BMDC),通过流式细胞术分析CD40和CD86在BMDC中的表达水平.进一步将VLPs、VLPs-CpG和VLPs-poly I∶C分别与ISA206乳化制备疫苗,并免疫豚鼠,通过检测特异性抗体、中和抗体、脾淋巴细胞增殖以及攻毒试验评估CpG和poly I∶C对VLPs疫苗免疫效果的影响.结果显示,VLPs-CpG组和VLPs-poly I∶C组的CD86表达水平高于VLPs组,ISA206佐剂联合poly I∶C、CpG均能提高攻毒保护率,而且ISA206联合CpG可以加速特异性抗体及中和抗体反应,提高脾淋巴细胞增殖水平.本研究结果表明,CpG和poly I∶C均能增强FMD VLPs疫苗的免疫效果,具有潜在的开发应用价值.
Abstract
This study was to evaluate the immune-enhancing effects of CpG and poly I∶C on the foot-and-mouth disease(FMD)virus-like particles(VLPs)vaccine.Using purified serotype 0 FMD VLPs as anti-gens,the mice bone marrow-derived dendritic cells(BMDC)were stimulated with VLPs-CpG and VLPs-poly I:C followed by the expression levels of CD40 and CD86 in BMDC was analyzed using flow cytometry.Fur-ther,VLPs,VLPs-CpG and VLPs-poly I∶C were emulsified with ISA206 to immunize guinea pigs.The improve-ment of CpG and poly I∶C on immune effect of the VLPs vaccine was evaluated by detecting the specific an-tibodies,neutralizing antibodies and splenic lymphocyte proliferation,as well as virus challenge.Re-sults showed that the expression levels of CD86 in VLPs-CpG and VLPs-poly I∶C were higher than VLPs.The adjuvant ISA206 combined with poly I∶C and CpG could improve the protection against virus.ISA206 com-bined with CpG accelerated the responses of specific antibodies and neutralizing antibodies,as well as enhanced the proliferation of splenic lymphocytes.This study demonstrates both CpG and poly I∶C has application potential to enhance the immune effect of FMD VLPs vaccine.
关键词
口蹄疫/病毒样颗粒/CpG/poly/I∶C/免疫效果Key words
foot-and-mouth disease(FMD)/virus-like particles(VLPs)/CpG/poly I∶C/immune efficacy引用本文复制引用
基金项目
国家肉牛牦牛产业技术体系项目(CARS-37)
国家重点研发计划项目(2021YFD1800300)
国家重点研发计划项目(2022YFD1800603)
国家自然科学基金项目(32072847)
国家自然科学基金项目(32072859)
国家自然科学基金项目(32301127)
兰州市人才创业创新项目(2023-RC-3)
甘肃省自然科学基金项目(22JR5RA032)
甘肃省自然科学基金项目(23JRRA551)
出版年
2024
国家科技期刊平台
NSTL
万方数据