Development of a quantum dot fluorescent microspheres immunochromatographic test strip for Brucella detection
To prepare a Brucella immunochromatographic strip based on quantum dot fluorescent microspheres,Brucella lipopolysaccharide monoclonal antibodies and goat anti-mouse IgG antibodies were sprayed onto nitrocellulose membranes as detection and quality control lines,respectively.Brucella lipopolysaccharide monoclonal antibodies were labeled with quantum dot fluorescent microspheres,diluted,and sprayed onto glass cellulose membranes.Finally,assembly and cutting were performed to produce test strips for detection,and their sensitivity,specificity,and stability were measured.Finally,using Brucella fluorescence quantitative PCR detection technology as a control,120 clinical samples were tested using this test strip to compare the compliance rates of the two methods.Results,the established quantum dot fluorescent microsphere immunochromatographic test strip showed good performance,with a detection sensitivity of 1 ng/mL for brucellosis and good specificity.There was no cross reaction with other pathogens.The stability test found that the product had good stability when stored at room temperature for 18 months or 37 ℃ for 28 d,with coefficients of variation of 3.2％and 14.6％,respectively;120 clinical samples were tested using test strips and fluorescence quantitative PCR methods.Compared with the results of fluorescence quantitative PCR,the positive conformity rate was 91％,the negative conformity rate was 100％,and the total conformity rate was 99％.Conclusion,the test strip has the advantages of simple operation,rap id stability,high sensitivity and low cost.The detection can be completed within 20 minutes,and the sample acquisition is easy.It can achieve rapid on-site detection and has good prospects in the pathogen detection and purification of brucellosis.
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