Establishment of a detection method for trichinellosis mediated by MnO2 nanosheets
This study focused on cystatin-like protein(CLP)as the research object,using the recom-binant protein rCLP prepared in the laboratory as the detection antigen for coating,and the anti-CLP monoclonal antibody(McAb)1H9 as the competitive antibody.Utilizing the unique optical properties of manganese dioxide nanosheets,an accurate,fast,and efficient colorimetric immunoassay for trichinello-sis was established.The specificity and sensitivity of this method were verified by using the serum of swine artificially infected with Trichinella spiralis,the serum of swine infected with other parasites,and the serum of swine immunized with conventional virus vaccines.The results showed that the serum of swine infected with Toxoplasma gondii,Cryptosporidium parvum,Cysticercus cellulosae,Ascaris suum,and Trichuris suis,and the sera of swine immunized with conventional virus vaccines(Vaccines for porcine pseudorabies virus,porcine reproductive and respiratory syndrome virus,porcine circovirus,swine fever virus,and foot-and-mouth disease virus)were all negative and no cross-reaction was observed.The serum samples of artificially infected pigs with three infection doses of different infection days were detected,including high(10 000 larvae/head),medium(1 000 larvae/head)and low(100 larvae/head)dose groups.The positive transformation time of serum antibody in the high-dose group was consistent with that of the commercial kit,and the positive transformation time of serum antibody in the medium and low dose groups was later than that of the commercial kit.At the same time,the positive detection rate of T.spiralis infection in the low dose group was higher than that of the artificial digestion method,indicating that the method was more sensitive.The established method was used for spiked recovery,the recovery rate was 98.8％—102.7％and the method was highly stable.The above results indicated that the proposed method for trichinellosis based on Mn02 nanosheets had high specificity,high sensitivity,simple operation,and can produce results without the large instruments.It can be used for on-site rapid screening of trichinellosis.
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