IBV promotes self-replication by inducing autophagy in Vero cells
In this study,to investigate whether avian Infectious bronchitis virus(IBV)can induce autophagy in cells and its effect on viral replication,DF-1 and Vero cells were inoculated with cell-adapted strain CEA of IBV.Then the expression and distribution of key proteins of autophagy path-way,LC3 and P62,and the formation of autophagosomes were detected by Western-blot,transmission elec-tron microscopy and confocal laser microscopy,respectively.The effect of autophagy on IBV replication was further evaluated by the treatment of autophagy inhibitor and inducer.The results showed that IBV did not induce LC3 transformation in DF-1 cells,but could increase levels of LC3-Ⅱ and P62 in Vero cells,as well as the formation of autophagosomes in Vero cells and the aggregation of LC3-Ⅱ into au-tophagosomes,suggesting that IBV could induce complete autophagy in Vero cells.The treatment with au-tophagy inhibitors,chloroquine and Wortmannin,inhibited IBV-induced autophagy and viral replication in Vero cells,meanwhile the treatment with autophagy activator rapamycin promoted viral replication.In summary,IBV could induce autophagy in Vero cells and promote viral replication.
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