Antibacterial susceptibility and resistance gene analysis of Klebsiella pneumoniae isolated from laying hens in Gansu and Ningxia
Klebsiella pneumoniae is an opportunistic pathogen that can carry and disseminate antibacterial resistance genes via mobile genetic elements.This study aimed to isolate and identify K.pneumoniae from laying hens in Gansu and Ningxia.Antimicrobial susceptibility testing and detection of resistance phenotypes and genotypes were performed using the disc diffusion test and PCR,respectively.From 245 samples,we isolated 54 strains of K.pneumoniae,resulting in an isolation rate of 22.04％.All 54 strains exhibited the ability to form biofilms.The isolated strains demonstrated significant resistance to antibacterials such as spectinomycin,trimethoprim-sulfamethoxazole,tetracycline,enrofloxacin and ofloxacin,with 92.59％displaying a multidrug-resistant phenotype.High detection rates of resistance genes,including qnrB(90.74％),blaSHV(85.19％),floR(75.93％),sul1(72.22％),gyrA(70.37％),and tetA(64.81％),were observed,correlating with the resistance phenotype.Furthermore,we discovered a strain of multidrug-resistant K.pneumoniae carrying extended-spectrum β-lactamase(ESBL)genes blaSHV,blaTEM,blaCTX-M,and colistin resistance genes mcr-1 andmcr-8.Additionally,eight other resistance genes were identified,suggesting a compilation of diverse resistance genes mediated by mobile genetic elements.The findings of the current study underscore a concerning prevalence of K.pneumoniae in laying hens in both Gansu and Ningxia,signifying a critical situation regarding multidrug resistance and a comprehensive array of resistance genes.
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