Identification and pathogen analysis of bovine respiratory disease complex
Bovine respiratory disease complex(BRDC)is a multi-symptom disease caused by various pathogens and environmental factors,resulting in significant economic losses for cattle farming industry.In March 2023,BRDC broke out in a beef cattle farm in Shanxi province.To detect the pathogens,4 swabs from the deep nasal cavity of infected cattle and 4 tissue samples from dead cattle were collected.These samples were detected by qPCR and RT-qPCR for 9 pathogens related to BRDC,includ-ing M.bovis,bovine parainfluenza virus type 3(BPIV-3),infectious bovine rhinotracheitis virus(I-BRV),bovine coronavirus(BCoV),bovine respiratory syncytial virus(BRSV),P.multocida,M.haemolyti-ca,Arcanobacterium pyogenes(A.pyogenes)and Histophilus somnus(H.somnus).The results indicated that the positive rate of M.bovis,P.multocida and H.somnus was 75％,the positive rate of M.haemolytica was 50％,A.pyogenes was 62％,the positive rates of BCoV were 25％.It was proved that the BRDC was caused by the mixed infection of the above 6 pathogens.The pathogens were isolated and identified to obtain 1 strain of M.hemolytia and 1 strain of P.multocida,and the drug susceptibility test was carried out.The results provided a reference for the prevention and control of calf respiratory diseases and the formu-lation of targeted treatment measures.
BRDCpathogen detectionmixed infectionpathogen isolation and identificationdrug sensitivity tests
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