Construction of recombinant pseudorabies virus expressing green fluorescent protein and its application in neutralization test
In order to optimize the viral serum neutralizing antibody test method and reduce the cycle time of traditional viral neutralizing antibody test,a pseudorabies gE gene deletion virus PRV-△gE-AcGFP1 expressing green fluorescent protein was constructed by homologous recombination technology,and used it as a test virus for determination of neutralizing antibody potency by performing the viral serum neutralizing antibody test on gE antibody-negative sera.The results showed that there was no significant difference between the recombinant virus and the traditional method in determining the titer of neutralizing antibody.The results show that PRV-△gE-AcGFP1 could be used as test virus for serum neutralizing antibody assay,providing a new option for performing convenient,accurate and short-cycle serum neutralizing antibody tests.
pseudorabies virushomologous recombinationgreen fluorescent proteinneutraliza-tion test
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