表达绿色荧光蛋白的重组伪狂犬病病毒构建及在中和试验中应用
Construction of recombinant pseudorabies virus expressing green fluorescent protein and its application in neutralization test
任晓祥 1刘立辉 2肖娜 2张帅 1武月 1赵云环 1刘涛 3左玉柱 1范京惠1
作者信息
- 1. 河北农业大学动物医学院,河北保定 071001
- 2. 定州市农业农村局,河北定州 073000
- 3. 瑞普(保定)生物药业有限公司,河北保定 071001
- 折叠
摘要
为了优化病毒血清中和抗体试验方法,减少传统病毒中和抗体试验的周期,本研究通过同源重组技术,构建出能够表达绿色荧光蛋白的伪狂犬gE基因缺失病毒PRV-△gE-AcGFP1.将其作为检测病毒,对gE抗体阴性血清进行病毒血清中和抗体试验,测定中和抗体效价.结果显示,利用重组病毒测定中和抗体效价的结果与传统方法无显著差异.结果表明,PRV-gE-AcGFP1可作为攻击病毒用于血清中和抗体试验,为进行方便、准确、周期短的血清中和抗体试验提供一种新的选择.
Abstract
In order to optimize the viral serum neutralizing antibody test method and reduce the cycle time of traditional viral neutralizing antibody test,a pseudorabies gE gene deletion virus PRV-△gE-AcGFP1 expressing green fluorescent protein was constructed by homologous recombination technology,and used it as a test virus for determination of neutralizing antibody potency by performing the viral serum neutralizing antibody test on gE antibody-negative sera.The results showed that there was no significant difference between the recombinant virus and the traditional method in determining the titer of neutralizing antibody.The results show that PRV-△gE-AcGFP1 could be used as test virus for serum neutralizing antibody assay,providing a new option for performing convenient,accurate and short-cycle serum neutralizing antibody tests.
关键词
伪狂犬病病毒/同源重组/绿色荧光蛋白/中和试验Key words
pseudorabies virus/homologous recombination/green fluorescent protein/neutraliza-tion test引用本文复制引用
基金项目
河北省农业产业技术体系生猪创新团队项目(HBCT2024220201)
河北省农业产业技术体系生猪创新团队项目(HBCT2024220401)
出版年
2024
国家科技期刊平台
NSTL
万方数据