Optimization of encapsulated conditions of Codonopsis pilosula polysaccharide PLGA nanoparticles using response surface methodology and their effects on RAW264.7
The aim of this experiment was to investigate the effects of poly lactic-co-glycolic acid(PLGA)loading on the structural characteristics and immune regulation of Codonopsis pilosula polysaccharide(CPP).Codonopsis pilosula polysaccharide PLGA nanoparticles(CPP-PLGA-NPs)were pre-pared by emulsified solvent volatilization method.The encapsulated conditions were optimized by the response surface method.The structures were analyzed by transmission electron microscopy,Zeta poten-tial and nanoparticle size analyzer.The immunoregulatory effects of CPP-PLGA-NPS and CPP on RAW264.7 cells were determined by MTT,flow cytometry and ELISA.The results show that the prepared nanoparticles have smooth surface,uniform morphology,good dispersion,stable state and small particle size.The op-timum preparation conditions were as follows:the ratio of organic phase to inner water phase was 8.51,the ratio of outer water phase to colostrum was 7.12,the concentration of F68 was 0.33％,the concen-tration of PLGA was 30 mg/mL,and the encapsulation rate of prepared nanoparticles was 93.54％±0.56％.Compared with blank group,CPP-PLGA-NPS and CPP significantly(P＜0.05)increased phagocytosis activi-ty of macrophages,secretion of NO,expression of CD40 and CD86,and secretion of cytokines(TNF-α,IL-6,IL-lβ).The regulatory effect of CPP-PLGA-NPs reached the highest at 0.97 μg/mL.The results showed that both CPP-PLGA-NPS and CPP had good immunomodulatory activity,which provided a theoretical basis for the development and utilization of Codonopsis pilosula polysaccharide.
国家科技期刊平台
NSTL
万方数据
