响应曲面法优化党参多糖PLGA纳米颗粒的包封条件及其对RAW264.7的影响
Optimization of encapsulated conditions of Codonopsis pilosula polysaccharide PLGA nanoparticles using response surface methodology and their effects on RAW264.7
王涛 1伍智天 1许逸舟 1郭劲航 1任喆 1秦韬1
作者信息
- 1. 福建农林大学动物科学学院福建省兽医中药与动物保健重点实验室,福建福州 350002
- 折叠
摘要
本试验旨在探寻聚乳酸一羟基乙酸共聚物(poly lactic-co-glycolic acid,PLGA)加载对党参多糖(Codonopsis pilosula polysaccharide,CPP)结构特征和免疫调控作用的影响.采用乳化溶剂挥发法制备党参多糖PLGA纳米颗粒(CPP-PLGA-NPs),应用响应曲面法优化包封条件,通过透射电镜、Zeta电位与纳米粒度仪分析其结构,并利用MTT法、流式细胞术、ELISA法测定CPP-PLGA-NPs和CPP对RAW264.7细胞的免疫调控作用.结果显示,制得的纳米颗粒表面光滑、形态均匀、分散性良好、状态稳定、粒径小.最佳制备条件为:有机相与内水相之比为8.51,外水相与初乳比为7.12,F68的浓度为0.33％,PLGA的浓度为30 mg/mL,制备的纳米颗粒的包封率为93.54％±0.56％.与空白组相比,CPP-PLGA-NPs和CPP都显著提高了巨噬细胞的吞噬活性、NO分泌量、CD40和CD86的表达量、细胞因子(TNF-α、IL-6、IL-1β)的分泌量(P＜0.05),在浓度为0.97 μg/mL时CPP-PLGA-NPs调控作用达到最高.结果表明,CPP-PLGA-NPs和CPP都有较好的免疫调节活性,为党参多糖的开发与利用提供了理论依据.
Abstract
The aim of this experiment was to investigate the effects of poly lactic-co-glycolic acid(PLGA)loading on the structural characteristics and immune regulation of Codonopsis pilosula polysaccharide(CPP).Codonopsis pilosula polysaccharide PLGA nanoparticles(CPP-PLGA-NPs)were pre-pared by emulsified solvent volatilization method.The encapsulated conditions were optimized by the response surface method.The structures were analyzed by transmission electron microscopy,Zeta poten-tial and nanoparticle size analyzer.The immunoregulatory effects of CPP-PLGA-NPS and CPP on RAW264.7 cells were determined by MTT,flow cytometry and ELISA.The results show that the prepared nanoparticles have smooth surface,uniform morphology,good dispersion,stable state and small particle size.The op-timum preparation conditions were as follows:the ratio of organic phase to inner water phase was 8.51,the ratio of outer water phase to colostrum was 7.12,the concentration of F68 was 0.33％,the concen-tration of PLGA was 30 mg/mL,and the encapsulation rate of prepared nanoparticles was 93.54％±0.56％.Compared with blank group,CPP-PLGA-NPS and CPP significantly(P＜0.05)increased phagocytosis activi-ty of macrophages,secretion of NO,expression of CD40 and CD86,and secretion of cytokines(TNF-α,IL-6,IL-lβ).The regulatory effect of CPP-PLGA-NPs reached the highest at 0.97 μg/mL.The results showed that both CPP-PLGA-NPS and CPP had good immunomodulatory activity,which provided a theoretical basis for the development and utilization of Codonopsis pilosula polysaccharide.
关键词
党参多糖/PLGA/响应曲面法/免疫调控Key words
Codonopsis pilosula polysaccharide/PLGA/response surface method/immune regulation引用本文复制引用
基金项目
福建省自然科学基金面上项目(2023J01074)
福建农林大学科技创新专项基金项目(KFB23102)
出版年
2024
国家科技期刊平台
NSTL
万方数据