首页|基于响应面法优化脂质纳米颗粒的配方及表征

基于响应面法优化脂质纳米颗粒的配方及表征

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  • 国家科技期刊平台
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脂质纳米颗粒是目前最有应用前景的药物载体之一,本研究旨在解决原材料配方对脂质纳米颗粒自组装的影响,优化其配方.首先,以包封率为评价指标,通过单因素试验确定膜材比、脂质浓度和DMG-PEG 2000 的含量.其次,采用Box-Benhnken响应面分析法优化脂质纳米颗粒制备工艺,并对其进行表征评价.结果表明,通过单因素试验确定的三因素分别是:膜材比、脂质浓度和DMG-PEG 2000含量.三因素的三水平分别为:膜材比选择1∶1、2∶1、3∶1,脂质浓度选择15、20、25mmol/L,DMG-PEG2000含量选择5％、7％、9％.通过Box-Benhnken响应面分析法得出:当脂质纳米颗粒的膜材比为2∶1,脂质浓度为20 mmol/L,DMG-PEG2000含量为7％时,其脂质纳米颗粒的包封率为(94.8±0.15)％,粒径为(43.77±0.25)nm,聚合物分散指数(polymer dispersity index,PD1)为(0.14±0.04),Zeta 电位为(-0.44±0.15)mV.利用透射电镜观察该脂质纳米颗粒的形态为球形结构、分散均匀.稳定性试验显示4 ℃放置28 d,脂质纳米颗粒的平均粒径、PDI和包封率无显著变化,说明该脂质纳米颗粒稳定性良好.本研究为制备一种高效的脂质纳米颗粒提供了参考依据.
Optimization and characterization of lipid nanoparticles based on response surface method
Lipid nanoparticles are one of the most promising drug carriers for application.To address the impact of raw material formulation on the self-assembly of lipid nanoparticles,the study aims to optimize its formulation.The encapsulation efficiency was used as the evaluation index,the film-material ratio,lipid concentration,and the content of DMG-PEG 2000 were determined by single factor experiments.Box-Benhnken response surface analysis was used to optimize the preparation process of lipid nanoparticles,and their characterization was evaluated.The results showed that the three factors film-material ratio,lipid concentration and DMG-PEG 2000 content were determined by single factor experiments.The three levels of the three factors were:film-material material ratio was selected as 1∶1 and 2∶1,3∶1,lipid concentration was selected as 15,20 and 25 mmol/L,and DMG-PEG2000 content was selected as 5％,7％and 9％.The results are obtained by Box-Benhnken response surface analysis.When the film-material ratio of lipid nanoparticles was 2∶1,the concentration of lipid was 20mmol/L,and the content of DMG-PEG2000 was 7％,the encapsulation efficiency of lipid nanoparticles was(94.8±0.15)％,the particle size was(43.77±0.25)nm,polymer dispersity index(PDI)was(0.14±0.04),Zeta potential was(-0.44±0.15)mV,and the shape of the lipid nanoparticle is spherical and uniformly dispersed by transmission electron microscop.The stability test showed that after being placed at 4 ℃ for 28 days,there is no significant change in the average particle size,PDI and encapsulation efficiency of the lipid nanoparticles,indicating good stability of the lipid nanoparticles.This study provides a reference basis for the preparation of an efficient lipid nanoparticle.

lipid nanoparticlesBox-Benhnken response surface methodsingle factorencapsula-tion efficiency

麻玉清、柳海云、郭伟、江丽馨、孙世琪、王晓强、马全英、郭慧琛

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兰州交通大学化学 化工学院,甘肃兰州 730070

中国农业科学院 兰州兽医研究所兰州大学动物医学与生物安全学院 动物疫病防控全国重点实验室,甘肃兰州 730046

兰州交通大学生物与制药工程学院,甘肃兰州 730070

脂质纳米颗粒 Box-Benhnken响应面法 单因素 包封率

国家重点研发计划项目国家重点研发计划项目国家自然科学基金项目国家自然科学基金项目国家自然科学基金项目国家自然科学基金青年基金项目2023兰州市人才创业创新项目

2022YFD18006032021YFD1800300320728593207284732002272323011272023-RC-3

2024

10.16656/j.issn.1673-4696.2024.0161

中国兽医科学
中国农业科学院兰州兽医研究所

中国兽医科学

CSTPCD北大核心
影响因子：0.524
ISSN：1673-4696
年,卷(期)：2024.54(10)
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