Isolation,identification and gene sequence analysis of bovine viral diarrhea virus NM-1 strain
To investigate the prevalence of bovine viral diarrhea and the genetic characteristics of BVDV epidemic strains in Inner Mongolia area,In this study,fecal samples of diarrheal calves from a dairy farm in Inner Mongolia area were collected,filtered and sterilized,and inoculated into bovine kidney cells(MDBK).After 5 generations of blind transmission,the cytopathic changes were observed and identified by RT-PCR.The results showed that a cytopathic bovine viral diarrhea strain was successfully isolated and named BVDV-NM-1 strain.The typical BVDV virion morphology of BVDV-NM-1 was observed by electron microscopy.The 5'-UTR gene sequence of BVDV-NM-1 strain was amplified and compared with that of BVDV-1 type.The homology of NM-1 strain with BVDV-1v subtype was 96％.It laid the foundation for better prevention and control of bovine viral diarrhea in Inner Mongolia area.
bovine viral diarrhea virus(BVDV)isolation and identificationsequence analysis
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