牛病毒性腹泻病毒NM-1株的分离鉴定及基因序列分析
Isolation,identification and gene sequence analysis of bovine viral diarrhea virus NM-1 strain
刘方圆 1张东亮 2吕文雪 3吕爽 3王春霞 3许皓 4袁帅 4陈雪 4徐凤宇 5赵传芳3
作者信息
- 1. 吉林农业大学动物医学院,吉林长春 130118;吉林特研生物技术有限责任公司,吉林长春 130022
- 2. 吉林农业大学动物医学院,吉林长春 130118
- 3. 中国农业科学院特产研究所,吉林长春 130021
- 4. 吉林特研生物技术有限责任公司,吉林长春 130022
- 5. 吉林农业大学动物医学院,吉林长春 130118;吉林农业大学动物生产及产品质量安全教育部重点实验室,吉林长春 130118
- 折叠
摘要
为探究内蒙古地区牛病毒性腹泻的流行情况及牛病毒性腹泻病毒(BVDV)流行株基因特征,本研究采集了内蒙古某奶牛场腹泻犊牛的粪便样品,经过滤除菌等处理后,接种于牛肾细胞(MDBK),盲传5代后,观察细胞病变并进行RT-PCR鉴定.结果显示,成功分离到1株能致细胞病变的BVDV毒株,命名为BVDV-NM-1株;电镜观察到BVDV-NM-1株具有典型BVDV病毒粒子形态;对BVDV-NM-1株5'-UTR基因序列扩增后进行序列比对,结果显示,BVDV-NM-1株属于BVDV-1型,与BVDV-1v亚型的基因同源性为96％.本研究结果为内蒙古地区牛病毒性腹泻的防控奠定了基础.
Abstract
To investigate the prevalence of bovine viral diarrhea and the genetic characteristics of BVDV epidemic strains in Inner Mongolia area,In this study,fecal samples of diarrheal calves from a dairy farm in Inner Mongolia area were collected,filtered and sterilized,and inoculated into bovine kidney cells(MDBK).After 5 generations of blind transmission,the cytopathic changes were observed and identified by RT-PCR.The results showed that a cytopathic bovine viral diarrhea strain was successfully isolated and named BVDV-NM-1 strain.The typical BVDV virion morphology of BVDV-NM-1 was observed by electron microscopy.The 5'-UTR gene sequence of BVDV-NM-1 strain was amplified and compared with that of BVDV-1 type.The homology of NM-1 strain with BVDV-1v subtype was 96％.It laid the foundation for better prevention and control of bovine viral diarrhea in Inner Mongolia area.
关键词
牛病毒性腹泻病毒/分离鉴定/序列分析Key words
bovine viral diarrhea virus(BVDV)/isolation and identification/sequence analysis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据