Effects of plasma-derived exosomes from Bactrian camel on the proliferation of HCC cells and its transcriptome level analysis
To explore the mechanism of the effect of Bactrian camel plasma-derived exosomes on the proliferation of hepatocellular carcinoma(HCC),the present study established an in situ tumor model of nude mice liver by utilizing a constructed fluorescent protein LUC-transfected cell line.Nude mice with HCC were randomly divided into control and experimental groups.And experimental group was divid-ed into normal Bactrian camel plasma-derived exosomes(N-Exo)and thin Bactrian camel plasma-derived exosomes(T-Exo)groups.The nude mice in each group were injected with PBS,N-Exo and T-Exo via tail vein for 3 weeks of intervention.After that,in vivo imaging system and HE staining methods were used to de-tect the effects of Bactrian camel plasma-derived exosomes on HCC cell proliferation.And the differ-ences between the two groups of exosomes at the transcriptome level were further analyzed.The results showed that in situ liver tumor model of nude mouse was successfully constructed using the screened stable MHCC-97H-LUC cell line.After treatment with Bactrian camel plasma-derived exosomes,the liver lobule structure of nude mice in T-Exo group disappeared,the tumor cells showed clumped and solid growth,and the fluorescence signal intensity of liver was higher,which was not significantly differ-ent from that of control group.The liver tissue of nude mice in the N-Exo group was morphologically nor-mal,with no inflammatory cell infiltration,and its fluorescence signal intensity was significantly decreased(P＜0.05).Transcriptome analysis revealed that a total of 40 differential miRNAs(DE-miRNAs)were identified in both exosomes,including miR-21-5p,miR-320c,miR-144-3p/5p,and others.These DE-miRNAs were significantly enriched in signaling pathways such as Ras,Rapl,and PI3K-Akt.The above results suggest that Bactrian camel plasma-derived exosomes may regulate the relevant signal ing path-ways through the above DE-miRNAs,thus affecting the proliferation of HCC cells.
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