Schisandra chinensis polysaccharide protects against liver injury in mice with DDS induced colitis
The study aimed to explore the protective effects of Schisandra chinensis polysaccharides(SCP)on liver injury induced by dextran sulfate sodium(DSS)in mice with colitis.Sixty 6-week-old male ICR mice were randomly divided into six groups,with 10 mice in each group:the control group(CON),the model group(DSS),the positive control group(5-aminosalicylic acid,5-ASA),the low-dose SCP group(DSS+50 mg/kg SCP),the medium-dose SCP group(DSS+100 mg/kg SCP),and the high-dose SCP group(DSS+200 mg/kg SCP).At the end of the experiment,the body weight and liver weight of the mice were measured,and the liver index was calculated.Blood and liver tissue were collected,and serum liver function biochemical indicators were measured using enzyme-linked immunosorbent assay(ELISA).Hematoxylin-eosin(HE)staining and Sirius red staining were performed on a portion of the liver tissue,while the rest of the liver tissue was used for detecting antioxidant activity,transaminase levels,inflammatory cytokine levels,and their Mrna expression.The results showed that compared to the control group,DSS treatment signifi-cantly reduced the liver weight and liver index of the mice(P＜0.01),caused pathological changes in the liver cell structure accompanied by inflammatory cell infiltration,increased collagen fiber levels in liver cells,decreased serum albumin(ALB),globulin(GLOB),and total protein(TP)levels(P＜0.05),increased serum and liver aspartate aminotransferase(AST)levels and the AST/ALT ratio(P＜0.01),increased liver malondialdehyde(MDA)levels(P＜0.01),decreased liver superoxide dismutase(SOD)and total antioxidant capacity(T-AOC)levels(P＜0.01),and increased levels of interleukin-6(IL-6),inter leukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α)in the liver(P＜0.01),as well as their Mrna expression(P＜0.05).Oral administration of medium and high doses of SCP significantly improved these changes in a dose-dependent manner.In conclusion,DSS induced structural damage,oxidative stress,and inflammation in the liver tissue of mice,and SCP had significant protective effects against these changes in a dose-dependent manner.
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