Study on the influence of wiping method on the bacteria content of output water from air-rotor handpiece con-nector of dental units
Objective To investigate the influence of wiping method on the bacteria content of output water from air-ro-tor handpiece connector of dental units.Methods The study was conducted from July to November 2022 at Cen-tral Laboratory,Peking University School and hospital of Stomatology.Twenty-four dental units with air-rotor hand-piece connectors that were used in daily outpatient clinics of oral and maxillofacial surgery were selected.Eight den-tal units were randomly selected,and samples were col-lected from air-rotor handpiece connectors and the output water 30 min before the opening of the clinic.After disinfect-ing the connectors with the wiping method,samples were taken again.The other 16 dental units were randomly divided into the study group and the control group(8 dental units in each group).The water flushing method was used for preoper-ative preparation in the control group,while the wiping plus water-flush method was used in the study group.The output water samples were collected from two groups after preoperative preparation.The experiments were repeated three times,with an interval of 14 days each time.The bacterial content of the samples were compared.The correlation between the bacterial content of the water samples and those of the connector samples were analyzed with Pearson correlation test.Results In the three replicate experiments,the bacterial content on the connector surface after disinfection by the wip-ing method was(795.8±1141.6),(1163.8±1124.0),(1173.4±960.1)CFU/mL,and the bacterial content of the output samples was(103.5±63.6),(94.1±71.7),(108.1±57.1)CFU/mL,respectively.Compared with the samples on the connector surface[(5459.4±4561.6),(9079.4±6602.5),(6605.0±3488.3)CFU/mL]and the output water sam-ples[(300.1±44.5),(324.0±167.4),(346.1±49.1)CFU/mL]before disinfection,a marked decrease was observed.All of the differences were statistically significant(all P＜0.05).Pearson correlation test showed a positive correlation between the bacterial content of the output water samples and that of the samples on the connector surface(r=0.789,P=0.020).In the three replicated experiments,the bacterial content on the connector surface from the study group was(94.5±93.0),(91.3±87.3),and(116.3±73.5)CFU/mL,and the bacterial content of the output water samples was(79.5±25.9),(78.1±25.4),and(65.9±24.6)CFU/mL,which were obviously lower than that from the control group[connector surface:(6318.0±1750.6),(6366.9±1672.5),(6321.6±1650.9)CFU/mL;output water:(308.9±124.5),(352.1±112.6),(298.1±98.7)CFU/mL].All of the differences were statistically significant(all P＜0.05).Conclusion There is a certain amount of bacteria at the air-rotor handpiece connectors of dental units,and the bacteri-al content is positively correlated with the bacterial content of the output water.Wiping and disinfecting the surface of the connector could effectively reduce the bacterial content of the output water.
air-rotor handpiece connectordental unit wate linemicroorganismsdisinfection
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