To estalish isolation and culture method of yak placental trophoblast cells,the present experiment was carried out by useing co-digestion of the feral placental tissues of 8 to 10 week old-fetus,to obtain the single trophoblast cell suspension.The trophoblast cells was then isolated and purified.The optimal concentration of FBS and optimal pH were determined for culturing the trophoblast cells;meanwhile,the general morphology and nuclear characteristics of the trophoblast cells,and the expression of cytokeratin7 (CK 7) and vimentin (Vim) were also examined.The results shown that the optimal pH for culturing trophoblast cell was 6.8-7.0 and the optimal medium was DMEM/ F12 with 20％ FBS.The cells were in shape of epithelial-like cell and growing in schistic spreading way,The immunohistochemistry staining showed that the cytokeratin chromosome was positive while the vimentin chromosome was negative.The positive cells rate amounted to 90％ and the cell viability was more than 95％ by trpan blue exclution.It was concluded that the used isolation methods and culture conditions in vitro might conveniently and effectively obtain high purified yak placental trophoblast cells.
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NSTL
维普
万方数据
