Isolation,identification and genomic sequencing analysis of CDV-ZC strain of canine distemper virus from raccoon dog
A virus strain was isolated by Vero cells from supernatant of cell extracts of liver,spleen,lung that were collected from a raccoon dog with suspected canine distemper virus infection in Zhucheng city,Shandong province.Typical cytopathic effect was observed in Vero cells after four passages.It was identified as CDV by virulence investigation,serology neutralization test,RT-PCR and animal regression experiment and named CDV Zhucheng strain.Full-length genome of strain CDV-ZC was amplified,sequenced and aligned with other CDV strains by RT-PCR,The completegenome nucleotide sequence of CDV ZC (KJ994343) exhibited highly homology to the standard virulent strain A75/17 (96,5％ at nt level),while it was identity with the vaccine strains Onderstepoort,CDV3 and so on ranged from 91.6％-92.0％.Phylogenetic analysis based on the hemagglutinin gene demonstrated that CDV-ZC strain belonged to Asia-1 geuotype,along with Chinese wild-type strain LN(13)2,GP and Japanese strain UENO,HAMA and so on.In inspecting the H protein alignment,a novel potential N-linked glycosylation sites was observed at position 542-544,which located at H protein receptor (SLAM) binding region.The successful isolation and complete sequences determination of highly pathogenic and virulent strain CDV-ZC provided a better understanding of the genetic variations of CDVs in China and is likely to be helpful to guide efficient preventive,diagnostic,and control strategies for CD.
canine distemper virusCDV-ZC strainisolation and identificationgenomic sequencehemagglutinin gene
NETL
NSTL
万方数据
维普
