All isolates(62) were tested for susceptibility to 7 different antimicrobial agents using broth micro-dilution method, and the presence of genes encoding these properties was tested.Furthermore, 16S rDNA of all isolates were screened and sequenced for molecular characterization.All isolates were susceptible to kanamycin, 93.5 ％ (58/62) for ciprofloxacin, gentamicin and ehloramphenicol,37.1％ (23/62) for tetracycline, 1.6 ％ (1/62) for erythromycin and vancomycin, respectively.All isolates were positive for aphA3, aadA, aadE, and aac6-aph2, followed by ermB (98.4％), tet(M) (98.4％), tet(O)(98.4％), aac (95.2％), mefA (88.7％), vanB (72.6％) ,and vanA (3.2 ％), respectively.However, catpIP gene was not found in the 62 isolates.Furthermore,phenotype of erythromycin-resistant was positively correlated with ermB(98.4％) and mefA (88.7 ％).Phenotype of chloramphenicol-resistant (6.5 ％) was well correlated with ca tpIP (0), and the phenotype of vancomycin-resistant(98.4％) was well correlated with vanB (73.6％) gene.While other genes have no obvious correlations between their genotype and phenotype.All 62 isolates were divided into 6 clusters,including A,B,C,D,E and F clusters.The homology of cluster E was closed to EU717958,and the homology of cluster F was closed to EU717964.
NETL
NSTL
维普
万方数据
